Thioflavin T Is a Fluorescent Probe of the Acetylcholinesterase Peripheral Site That Reveals Conformational Interactions between the Peripheral and Acylation Sites

Journal of Biological Chemistry

Volumn 276, Issue 26, 2001, Pages 23282-23287

  De Ferrari, Giancarlo V ^b   Mallender, William D ^a,c   Inestrosa, Nibaldo C ^b   Rosenberry, Terrone L ^a  

^a MAYO CLINIC   (United States)

^b PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

^c MILLENNIUM PHARMACEUTICALS INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLATION; CONFORMATIONS; DISSOCIATION; FLUORESCENCE; MOLECULAR STRUCTURE; QUENCHING;

LIGAND BINDING;

ENZYMES;

ACETYLCHOLINESTERASE; EDROPHONIUM; LIGAND; RECOMBINANT ENZYME; THIOFLAVINE; ACETOPHENONE DERIVATIVE; CHOLINESTERASE INHIBITOR; COLORING AGENT; FLUORESCENT DYE; M (N,N,N TRIMETHYLAMMONIO)TRIFLUOROACETOPHENONE; M-(N,N,N-TRIMETHYLAMMONIO)TRIFLUOROACETOPHENONE; PROPIDIUM IODIDE; THIAZOLE DERIVATIVE;

ACYLATION; ARTICLE; CATALYSIS; CHEMICAL STRUCTURE; DISSOCIATION CONSTANT; ENZYME PURIFICATION; ENZYME SUBSTRATE; FLUORESCENCE; LIGAND BINDING; NEUROTRANSMISSION; PRIORITY JOURNAL; STEADY STATE; BINDING SITE; CHEMISTRY; DOSE RESPONSE; HUMAN; METABOLISM; PROTEIN CONFORMATION;

ACETOPHENONES; ACETYLCHOLINESTERASE; ACYLATION; BINDING SITES; CHOLINESTERASE INHIBITORS; COLORING AGENTS; DOSE-RESPONSE RELATIONSHIP, DRUG; EDROPHONIUM; FLUORESCENT DYES; HUMANS; PROPIDIUM; PROTEIN CONFORMATION; THIAZOLES;

EID: 0035968202     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M009596200     Document Type: Article

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