The DNA polymerase III holoenzyme: An asymmetric dimeric replicative complex with leading and lagging strand polymerases

Cell

Volumn 105, Issue 7, 2001, Pages 925-934

  Glover, Bradley P ^a   McHenry, Charles S ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE DERIVATIVE; DNA BINDING PROTEIN; DNA DIRECTED DNA POLYMERASE GAMMA; DNA POLYMERASE; SINGLE STRANDED DNA;

ANALYTIC METHOD; ARTICLE; COMPLEX FORMATION; DNA REPLICATION; DNA STRAND; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME STRUCTURE; PRIORITY JOURNAL; REGULATORY MECHANISM;

EID: 0035967930     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(01)00400-7     Document Type: Article

References (38)

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3. DnaX complex of Escherichia coli DNA polymerase III holoenzyme. Central role of τ in initiation complex assembly and in determining the functional asymmetry of holoenzyme
4. The χψ subunits of DNA polymerase III holoenzyme bind to single-stranded DNA-binding protein (SSB) and facilitate replication of an SSB-coated template
5. The DnaX-binding subunits δ′ and ψ are bound to γ and not τ in the DNA polymerase III holoenzyme
6. Glutamate overcomes the salt inhibition of DNA polymerase III holoenzyme
7. Monoclonal antibodies specific for the τ subunit of the DNA polymerase III holoenzyme of Escherichia coli. Use to demonstrate that τ is the product of the dnaZX gene and that both it and γ, the dnaZ gene product, are integral components of the same enzyme assembly
8. ATP binding to the Escherichia coli clamp loader powers opening of the ring-shaped clamp of DNA polymerase III holoenzyme
9. Adenosine 5′-O-(3-thiotriphosphate) can support the formation of an initiation complex between the DNA polymerase III holoenzyme and primed DNA
10. Chemical characterization and purification of the β subunit of the DNA polymerase III holoenzyme from an overproducing strain
11. Devoted to the lagging strand-the χ subunit of DNA polymerase III holoenzyme contacts SSB to promote processive elongation and sliding clamp assembly
12. In vivo assembly of overproduced DNA polymerase III. Overproduction, purification, and characterization of the α, α-ε, and α-ε-θ subunits
13. τ Couples the leading- and lagging-strand polymerases at the Escherichia coli DNA replication fork
14. Coupling of a replicative polymerase and helicase: A τ-DnaB interaction mediates rapid replication fork movement
15. τ Protects β in the leading-strand polymerase complex at the replication fork
16. The dnaX gene encodes the DNA polymerase III holoenzyme τ subunit, precursor of the γ subunit, the dnaZ gene product
17. The Escherichia coli dnaB replication protein is a DNA helicase
18. Two distinct triggers for cycling of the lagging strand polymerase at the replication fork
19. DNA Polymerase III holoenzyme of Escherichia coli. IV. The holoenzyme is an asymmetric dimer with twin active sites
20. Purification and characterization of DNA polymerase III′. Identification of τ as a subunit of the DNA polymerase III holoenzyme
21. DNA polymerase III holoenzyme of Escherichia coli
22. The asymmetric dimeric polymerase hypothesis: A progress report
23. Replication of pBR322 DNA in vitro with purified proteins. Requirement for topoisomerase I in the maintenance of template specificity
24. Cloning of the escherichia coli dnaZX region and identification of its products
25. Discontinuous DNA replication
26. DNA polymerase III accessory proteins. II. Characterization of δ and δ′
27. Assembly of a chromosomal replication machine: Two DNA polymerases, a clamp loader, and sliding clamps in one holoenzyme particle. III. Interface between two polymerases and the clamp loader
28. 3δδ′
29. Escherichia coli DNA polymerase III holoenzyme footprints three helical turns of its primer
30. The γε-c subunit interface in the ATP synthase of Escherichia coli. Cross-linking of the ε subunit to the c subunit ring does not impair enzyme function, that of γ to c subunits leads to uncoupling
31. Purification and characterization of a mutant DnaB protein specifically defective in ATP hydrolysis
32. Efficient in vitro replication of double-stranded DNA templates by a purified T4 bacteriophage replication system
33. The rotary mechanism of ATP synthase
34. Constitution of the twin polymerase of DNA polymerase III holoenzyme
35. Mechanism of DNA elongation catalyzed by Escherichia coli DNA polymerase III, dnaZ protein, and DNA elongation factors I and III
36. Replisome assembly reveals the basis for asymmetric function in leading and lagging strand replication
37. Trading places on DNA - A three-point switch underlies primer handoff from primase to the replicative DNA polymerase