Conjugating oligosaccharides to proteins by squaric acid diester chemistry: Rapid monitoring of the progress of conjugation, and recovery of the unused ligand

Carbohydrate Research

Volumn 330, Issue 4, 2001, Pages 479-486

  Chernyak, Anatoly ^a   Karavanov, Alex ^b   Ogawa, Yuji ^a   Kováč, Pavol ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b CIPHERGEN BIOSYSTEMS INC   (United States)

Author keywords

Molecular mass determination; Neoglycoconjugate; SELDI TOF MS ProteinChip ; Squaric acid derivatives; Vibrio cholerae O:1

Indexed keywords

CARRIER PROTEIN; LIGAND; OLIGOSACCHARIDE; SQUARIC ACID DERIVATIVE;

ARTICLE; CHEMICAL REACTION; CONJUGATION; MASS SPECTROMETRY; MOLECULAR WEIGHT; MONITORING; PRIORITY JOURNAL; REACTION ANALYSIS; TECHNIQUE;

ANIMALS; CARBOHYDRATE CONFORMATION; CARBOHYDRATE SEQUENCE; CATTLE; CYCLOBUTANES; ESTERS; GLYCOPROTEINS; GLYCOSYLATION; LIGANDS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; O ANTIGENS; OLIGOSACCHARIDES; SERUM ALBUMIN, BOVINE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; VIBRIO CHOLERAE;

VIBRIO CHOLERAE;

EID: 0035961431     PISSN: 00086215     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0008-6215(01)00018-0     Document Type: Article

References (24)