Identification of six chymotrypsin cDNAs from larval midguts of Helicoverpa zea and Agrotis ipsilon feeding on the soybean (Kunitz) trypsin inhibitor

Insect Biochemistry and Molecular Biology

Volumn 31, Issue 6-7, 2001, Pages 633-644

  Mazumdar Leighton, Sudeshna ^a   Broadway, Roxanne M ^a  

^a New York State Agricultural Experiment Station   (United States)

Author keywords

Chymotrypsin; Herbivory; Introns; Lepidoptera; Midgut mRNA; Molecular biology; Promoters; Proteinase inhibitors

Indexed keywords

CHYMOSTATIN; CHYMOTRYPSIN; COMPLEMENTARY DNA; PROTEINASE INHIBITOR; SOYBEAN TRYPSIN INHIBITOR; TRYPSIN INHIBITOR;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CONSENSUS SEQUENCE; DIET; DIGESTIVE SYSTEM; DNA DETERMINATION; DNA LIBRARY; DNA SEQUENCE; EXON; FEEDING BEHAVIOR; GENE AMPLIFICATION; GENE CONTROL; INTRON; LARVA; LEPIDOPTERA; MIDGUT; MOLECULAR GENETICS; MOTH; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; SEQUENCE ANALYSIS; SOUTHERN BLOTTING; TATA BOX;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; CHYMOTRYPSIN; DIGESTIVE SYSTEM; DNA, COMPLEMENTARY; EXONS; FEEDING BEHAVIOR; GENE AMPLIFICATION; INTRONS; LARVA; MOLECULAR SEQUENCE DATA; MOTHS; SEQUENCE ANALYSIS, DNA; TRYPSIN INHIBITOR, KUNITZ SOYBEAN; TRYPSIN INHIBITORS;

AGROTIS IPSILON; GLYCINE MAX; HELICOVERPA ZEA; HEXAPODA; INSECTA; LEPIDOPTERA; MAMMALIA; ZEA;

EID: 0035957677     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(00)00168-5     Document Type: Article

References (48)

1. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
2. Isolation and sequence of a rat chymotrypsin B gene
3. Identity of the tryptic and α-chymotryptic reactive sites on the soybean trypsin inhibitor (Kunitz)
4. The structure of an insect chymotrypsin
5. Differentially regulated inhibitor-sensitive and insensitive protease genes from the phytophagous insect pest, Helicoverpa armigera, are members of complex multigene family
6. Are insects resistant to plant proteinase inhibitors?
7. Dietary proteinase inhibitors alter complement of midgut proteases
8. Dietary regulation of serine proteinases that are resistant to serine proteinase inhibitors
9. Splice junctions: Association with variation in protein structure
10. A gene family in Drosophila melanogaster coding for trypsin-like enzymes
11. Characterization of major midgut proteinase cDNAs from Helicoverpa armigera larvae and change in gene expression in response to four proteinase inhibitors in diet
12. Digestive proteolytic activity in larvae of tomato moth, Lacanobia oleracea: Effects of plant protease inhibitors in vivo and in vitro
13. Chickpea defensive proteinase inhibitors can be inactivated by podborer gut proteinases
14. Chymotrypsin
15. Comparative modelling methods: Application to the family of mammalian serine proteases
16. Databases on transcriptional regulation: TRANSFAC, TRRD and COMPEL
17. Isolation of high molecular weight DNA from insects
18. Raw soy and purified proteinase inhibitors induce the appearance of inhibitor-resistant trypsin and chymotrypsin activities in Wistar rat duodenal juice
19. Amino acid sequence of an insect chymotrypsin from the larvae of the hornet, Vespa orientalis
20. Multiple sequence alignment with CLUSTAL X
21. Adaptation of Spodoptera exigua larvae to plant proteinase inhibitors by induction of gut proteinase activity insensitive to inhibition
22. Protease activities in the larval midgut of Heliothis virescens: Evidence for Trypsin and chymotrypsin-like enzymes
23. Interactions of insect digestive enzymes with plant protein inhibitors and host-parasite co-evolution
24. Cloning and sequencing of a cDNA for a highly basic protease from the digestive juice of the silkworm, Bombyx mori
25. Isolation and characterization of two chymotrypsins from the midgut of Locusta migratoria
26. Endoproteases from the midgut of larval Spodoptera littoralis include a chymotrypsin-like enzymes with an extended binding site
27. Opposite effects of Spodoptera littoralis larvae of high expression level of a trypsin proteinase inhibitor in transgenic plants
28. Identification of novel serine proteinase gene transcripts in the midguts of two tropical insect pests, Scirpophaga incertulas (Wk.) and Helicoverpa armigera (Hb.)
29. Transcriptional induction of diverse midgut trypsins in larval Agrotis ipsilon and Helicoverpa zea feeding on the STI
30. Evolution of structure and function of proteases
31. Limited proteolysis and zymogen activation
32. Structural basis for substrate specificity in the serine proteases
33. Purification, characterization and cDNA sequence of an alkaline chymotrypsin from the midgut of Manduca sexta
34. The developmental regulation of amylolytic and proteolytic enzymes in the embryonic rat pancreas
35. Role of the S′ subsites in serine protease catalysis. Active-site mapping of rat chymotrypsin, rat trypsin, a-lytic protease, and cercarial protease from Schistosoma mansoni
36. Substrate binding site in bovine chymotrypsin Aγ a crystallographic study using peptide chloromethyl ketones as site specific inhibitors
37. Polymorphism and evolution of collagenolytic serine protease genes in crustaceans
38. Splice junctions, branch point sites and exons: Sequence statistics, identifications, and applications to genome project
39. Processing of δ-endotoxin of Bacillus thuringiensis subsp. Kurstaki HD-1 in Heliothis armigera midgut juice and the effects of protease inhibitors
40. Nuclear factor recognition sites in the gut-specific enhancer region of an Anopheles gambiae trypsin gene
41. Chymotrypsin C.
42. Digestive enzymes
43. Purification and characterization of the western spruce budworm larval midgut proteinases and comparision of gut activities of laboratory-reared and field-collected insects
44. Attempts to convert chymotrypsin to trypsin
45. Genomic organization and expression of a trypsin gene from the spruce budworm, Choristoneura fumiferana
46. Adaptation of Helicoverpa armigera (Lepidoptera:Noctuidae) to proteinase inhibitor expressed in transgenic tobacco
47. cDNAs for a chymotrypsinogen-like protein from two strains of Plodia interpunctella