Isolation and characterisation of two chymotrypsins from the midgut of Locusta migratoria

Insect Biochemistry and Molecular Biology

Volumn 29, Issue 7, 1999, Pages 653-660

  Lam, Winnie ^a   Coast, Geoffrey M ^a   Rayne, Richard C ^a  

^a UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Chymotrypsin; Endopeptidase; Locust; Locusta migratoria; Midgut; Protease

Indexed keywords

4 (2 AMINOETHYL)BENZENESULFONYL FLUORIDE; ANILIDE; APROTININ; BENZYLSULFONYL FLUORIDE; CHYMOSTATIN; CHYMOTRYPSIN; SERINE PROTEINASE; SOYBEAN TRYPSIN INHIBITOR; TOSYLLYSYL CHLOROMETHYL KETONE; TOSYLPHENYLALANYL CHLOROMETHYL KETONE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME KINETICS; ENZYME PURIFICATION; LOCUST; MALE; MASS SPECTROMETRY; MICHAELIS CONSTANT; MIDGUT; NONHUMAN;

EID: 0032792920     PISSN: 09651748     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0965-1748(99)00049-1     Document Type: Article

References (33)

1. Aoyagi T., Umezawa H. Structures and activities of protease inhibitors of microbial origin. Reich E., Rifkin D.B., Shaw E. Proteases and Biological Control. 1975;429-454 Cold Spring Harbor, New York.
2. Baumann E. Isolation and partial characterization of a chymotrypsin-like endoprotease from cockroach intestinal system. Insect Biochem. 20:1990;761-768.
3. Blum H., Beier H., Gross H.J. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis. 8:1987;93-99.
4. Bown D.P., Wilkinson H.S., Gatehouse J.A. Differentially regulated inhibitor-sensitive and insensitive protease genes from the phytophagous insect pest, Helicoverpa armigera, are members of complex multigene families. Insect Biochem. Molec. Biol. 27:1997;625-638.
5. Christeller J.T., Shaw B.D., Gardiner S.E., Dymock J. Partial purification and characterization of the major midgut proteases of grass grub larvae (Costelytra zealandica Coleoptera: scarabaeidae). Insect Biochem. 19:1989;221-231.
6. Christeller J.T., Laing W.A., Markwick N.P., Burgess E.P.J. Midgut protease activities in 12 phytophagous lepidopteran larvae: dietary and protease inhibitor interactions. Insect Biochem. Molec. Biol. 22:1992;735-746.
7. Cook R.G. Amino acid sequence analysis of blotted proteins. Dunbar B.S. Protein blotting, a practical approach. 1994;207-220 Oxford University Press, Oxford.
8. Davis C.A., Riddell D.C., Higgins M.J., Holden J.J.A., White B.N. A gene family in Drosophila melanogaster coding for trypsin-like enzymes. Nucl. Acids Res. 13:1985;6605-6619.
9. Gatehouse L.N., Shannon A.L., Burgess E.P.J., Christelller J.T. Characterization of major midgut proteinase cDNAs from Helicoverpa armigera larvae and changes in gene expression in response to four proteinase inhibitors in the diet. Insect Biochem. Molec. Biol. 27:1997;929-944.
10. Graf R., Boehlen P., Briegel H. Structural diversity of trypsin from different mosquito species feeding on vertebrate blood. Experientia. 47:1991;603-609.
11. Houseman J.G., Downe A.E.R. Cathepsin D-like activity in the posterior midgut of hemipteran insects. Comp. Biochem. Physiol. 75B:1983;509-512.
12. Houseman J.G., Morrison P.E., Downe A.E.R. Cathepsin B and aminopeptidase in the posterior midgut of Phymata wolffii (Hemiptera: Phymatidae). Can. J. Zool. 63:1985;1288-1291.
13. Jany K.-D., Haug H., Pfleiderer G., Ishay J. Enzymatic and chemical properties of an endopeptidase from the larva of the hornet Vespa crabro. Biochemistry. 17:1978;4675-4682.
14. Jany K.-D., Bekelar K., Pfleiderer G., Ishay J. Amino acid sequence of an insect chymotrypsin from the larvae of the hornet, Vespa orientalis. Biochem. Biophys. Res. Commun. 110:1983;1-7.
15. Jiang Q., Hall M., Noriega F.G., Wells M. cDNA cloning and pattern of expression of an adult, female-specific chymotrypsin from Aedes aegypti midgut. Insect Biochem. Molec. Biol. 27:1997;283-289.
16. Johnston K.A., Lee M.J., Gatehouse J.A., Anstee J.H. The partial purification and characterisation of serine protease activity in midgut of larval Helicoverpa armigera. Insect Biochem. 21:1991;389-397.
17. Johnston K.A., Lee M.J., Brough C., Hilder V.A., Gatehouse A.M.R., Gatehouse J.A. Protease activities in the larval midgut of Heliothis virescens: Evidence for trypsin and chymotrypsin-like enzymes. Insect Biochem. Molec. Biol. 25:1995;375-383.
18. Kalhok S.E., Tabak L.M., Prosser D.E., Brook W., Downe A.E.R., White B.N. Isolation, sequencing and characterization of two cDNA clones coding for trypsin-like enzymes from the midgut of Aedes aegypti. Insect Molec. Biol. 2:1993;71-79.
19. Klein U., Kock A., Moffett D.F. Ion transport in Lepidoptera. Lehane M.J., Billingsley P.F. Biology of the Insect Midgut. 1996;236-264 Chapman and Hall, London.
20. Knecht M., Hagenmaier H.E., Zebe E. The proteases in the gut of the locust, Locusta migratoria. J. Insect Physiol. 20:1974;461-470.
21. 4. Nature (London). 227:1970;680-685.
22. Lee M.J., Anstee J.H. Endoproteases from the midgut of larval Spodoptera littoralis include a chymotrypsin-like enzyme with an extended binding site. Insect Biochem. Molec. Biol. 25:1995;49-61.
23. Muller H.-M., Crampton J.M., della Torre A., Sinden R., Crisanti A. Members of a trypsin gene family in Anopheles gambiae are induced in the gut by blood meal. EMBO J. 12:1993;2891-2900.
24. Murdock L.L., Brookhart G., Dunn P.E., Foard D.E., Kelly S., Kitch L., Shade R.E., Shukle R.H., Wolfson J.L. Cysteine digestive proteinases in Coleoptera. Comp. Biochem. Physiol. 87B:1987;783-787.
25. Pearson W.R., Lipman D.J. Improved tools for biological sequence analysis. Proc. Natl. Acad. Sci. 85:1988;2444-2448.
26. Peterson A.M., Barillas-Mury C.V., Wells M.A. Sequence of three cDNAs encoding an alkaline midgut trypsin from Manduca sexta. Insect Biochem. Molec. Biol. 24:1994;463-471.
27. Peterson A.M., Fernando G.J.P., Wells M.A. Purification, characterization and cDNA sequence of an alkaline chymotrypsin from the midgut of Manduca sexta. Insect Biochem. Molec. Biol. 25:1995;765-774.
28. Sakal E., Applebaum S.W., Birk Y. Purification and characterization of Locusta migratoria chymotrypsin. Int. J. Pept. Prot. Res. 32:1988;590-598.
29. Sakal E., Applebaum S.W., Birk Y. Purification and characterization of trypsins from the digestive tract of Locusta migratoria. Int. J. Pept. Prot. Res. 34:1989;498-505.
30. Schechter I., Berger A. On the size of the active site in proteases I. Papain. Biochem. Biophys. Res. Comm. 27:1967;157-162.
31. Terra W.R., Ferreira C. Insect digestive enzymes: properties, compartmentalization and function. Comp. Biochem. Physiol. 109B:1994;1-62.
32. Terra W.R., Ferreira C., Jordão B.P., Dillon R.J. Digestive enzymes. Lehane M.J., Billingsley P.F. Biology of the Insect Midgut. 1996;153-194 Chapman and Hall, London.
33. Wolfson J.L., Murdock L.L. Diversity in digestive proteinase activity among insects. J. Chem. Ecol. 16:1990;1089-1102.