Quantitation of soluble and skeletal alkaline phosphatase, and insoluble alkaline phosphatase anchor-hydrolase activities in human serum

Clinica Chimica Acta

Volumn 311, Issue 2, 2001, Pages 137-148

  Anh, D J ^a   Eden, A ^a   Farley, J R ^a,b,c  

^a Jerry L Pettis Memorial VA Medical Center   (United States)

^b LOMA LINDA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

ALP anchor hydrolase; Anchorless form; Human serum

Indexed keywords

ALKALINE PHOSPHATASE; ALKALINE PHOSPHATASE ANCHOR HYDROLASE; ALKALINE PHOSPHATASE ISOENZYME; BILE SALT; LIVER ENZYME; MUSCLE ENZYME; UNCLASSIFIED DRUG;

ARTICLE; BLOOD ANALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME LOCALIZATION; ENZYME PURIFICATION; FEMALE; HEAT; HUMAN; HUMAN CELL; LIPID BLOOD LEVEL; MAJOR CLINICAL STUDY; PH; POSTMENOPAUSE; PRIORITY JOURNAL; TEMPERATURE;

ALKALINE PHOSPHATASE; BONE AND BONES; FEMALE; HUMANS; ISOENZYMES; KINETICS; LIVER; MIDDLE AGED; PHOSPHOLIPASE D; SOLUBILITY;

EID: 0035949770     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-8981(01)00584-8     Document Type: Article

References (55)

1. Alkaline phosphatase isozymes: What we know and what we don't know
2. Alkaline phosphatase isozymes: Recent progress
3. Perspectives in alkaline phosphatase research
4. Cell surface anchoring of proteins via glycosyl-phosphatidyl-inositol structures
5. Alkaline phosphatase cDNA transfected cells promote calcium and phosphorous deposition
6. A missense mutation in the human liver/bone/kidney alkaline phosphatase gene causing a lethal form of hypophosphatasia
7. Alkaline phosphatase knock-out mice recapitulate the metabolic and skeletal defects of infantile hypophosphatasia
8. Inactivation of two mouse alkaline phosphatase genes and establishment of a model of infantile hypophosphatasia
9. 6
10. Functional characterization of osteoblasts and osteoclasts from alkaline phosphatase knockout mice
11. Bone remodeling and calcium metabolism: A correlated histomorphometric, calcium kinetic, and biochemical study in patients with osteoporosis and Paget's disease
12. Skeletal alkaline phosphatase activity as a bone formation index in vitro
13. Bone-alkaline phosphatase as indicator of bone formation
14. Human liver plasma membranes contain an enzyme activity that removes membrane anchor from alkaline phosphatase and converts it to a plasma-like form
15. The autorelease of alkaline phosphatase from the plasma membrane during the incubation of cultured liver cell homogenates
16. Human osteosarcoma cells spontaneously release matrix-vesicle-like structures with the capacity to mineralize
17. Matrix vesicles produced by osteoblast-like cells in culture become significantly enriched in proteoglycan-degrading metalloproteinases after addition of β-glycerolphosphate and ascorbic acid
18. Skeletal alkaline phosphatase activity is primarily released from human osteoblasts in an insoluble form, and the net release is inhibited by calcium and skeletal growth factors
19. Osteoblast programmed cell death (apoptosis): Modulation by growth factors and cytokines
20. Molecular biology of matrix vesicles
21. Active role of chondrocyte apoptosis in endochondral ossification
22. Biology of human alkaline phosphatases with special reference to cancer
23. Secretion of hepatic and intestinal alkaline phosphatases: Similarities and differences
24. A glycan-phosphatidylinositol-specific phospholipase D in human serum
25. Purification of a phosphatidylinositol-glycan-specific phospholipase C from liver plasma membranes: A possible target of insulin action
26. Alkaline phosphatase releasing activity in human tissues
27. Primary structure and functional activity of a phosphatidylinositol-glycan-specific phospholipase D
28. Glycosylphosphatidylinositol-specific phopholipase D
29. Purification and characterization of glycosyl-phosphatidylinositol-specific phospholipase D
30. Purification of a glycosyl-phosphatidylinositol-specific phospholipase D from human plasma
31. Tetrameric alkaline phosphatase from human liver is converted to dimers by phosphatidylinositol phospholipase C
32. Glycosyl-phosphatidylinositol anchored membrane enzymes
33. Structural and functional roles of glycosyl-phosphatidylinositol in membranes
34. Factors affecting the ability of glycosylphosphatidylinositol-specific phospholipase D to degrade membrane anchors of cell surface proteins
35. The glycosyl-phosphatidylinositol anchor of membrane proteins
36. Hydrolysis of membrane-bound liver alkaline phosphatase by GPI-PLD requires bile salts
37. Generation by limited proteolysis of a catalytically active 39-kDa protein from the 115-kDa form of phosphatdiylinositol-glycan-specific phospholipase D from bovine serum
38. Phosphatidylinositol-glycan-specific phospholipase D is an amphiphilic glycoprotein that is associated with high-density lipoproteins
39. Enzymatic properties of phosphatidylinositol-glycan-specific phospholipase C from rat liver and phoshatidylinositol-glycan-specific phospholipase D from rat serum
40. A method for the assay of phosphatidylinositol-specific phospholipase D activity in serum
41. Alkaline phosphatase isoforms in bile and serum and their generation from cells in vitro
42. Inositol-specific phospholipase D activity in health and disease
43. Physicochemical and pathophysiological factors in the release of membrane-bound alkaline phosphatase from cells
44. Conversion of glycosylphosphatidylinositol (GPI)-anchored alkaline phosphatase by GPI-PLD
45. Glycosylphosphatidylinositol-specific phospholipase D of human serum - Activity modulation by naturally occurring amphiphiles
46. Glycosylphosphatidylinositol-specific phospholipase D in blood serum: Is the liver the only source of the enzyme?
47. Solubilization of liver alkaline phosphatase isoenzyme during cholestasis in dogs
48. Quantification of skeletal alkaline phosphatase in osteoporotic serum by wheat germ agglutinin precipitation, heat inactivation, and a two-site immunoradiometric assay
49. A simplified heat-inactivation method for investigating alkaline phosphatase isoenzymes in serum
50. Alkaline phosphatase activity from human osteosarcoma cell line SaOS-2: An isoenzyme standard for quantifying skeletal alkaline phosphatase activity in serum
51. Reference standards for quantification of skeletal ALP activity in serum by heat inactivation and lectin precipitation
52. Phase separation of integral membrane proteins in Triton X-114 solution
53. A rapid and sensitive method for quantitation of microgram amounts of protein using the principle of protein-dye binding
54. Matrix vesicle calcification in bones of adult rats