Identification of genotoxic stress in human cells by fluorescent monitoring of p53 expression

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 494, Issue 1-2, 2001, Pages 73-85

  Quiñones, Ariel ^a   Rainov, Nikolai G ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Carcinogens; DNA damage; EGFP; Genotoxic stress; Mutagens; p53

Indexed keywords

2 AMINOPURINE; 4 NITROQUINOLINE 1 OXIDE; AFLATOXIN B1; AZATHIOPRINE; BENZO[A]PYRENE; CARMUSTINE; CISPLATIN; DIMETHYL SULFOXIDE; DOXORUBICIN; EPIDERMAL GROWTH FACTOR; HYDROGEN PEROXIDE; MESYLIC ACID METHYL ESTER; METHYLNITRONITROSOGUANIDINE; MITOMYCIN C; PACLITAXEL; PHENOBARBITAL; PROTEIN P53; RESVERATROL; SACCHARIN; TOPOTECAN; VIRUS PROTEIN;

ARTICLE; BIOACCUMULATION; CELL LEVEL; CELLULAR DISTRIBUTION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DAMAGE; GENE EXPRESSION; GENE FUSION; GENE INDUCTION; GENETIC TRANSFECTION; GENOTOXICITY; HUMAN; HUMAN CELL; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN LOCALIZATION; RNA TRANSLATION;

4-NITROQUINOLINE-1-OXIDE; CARCINOGENICITY TESTS; DNA DAMAGE; DOXORUBICIN; GENE EXPRESSION; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; METHYL METHANESULFONATE; MITOMYCIN; MUTAGENICITY TESTS; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0035948802     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(01)00179-6     Document Type: Article

References (53)

1. p53, the cellular gatekeeper for growth and division
2. The complexity of p53 modulation: Emerging patterns from divergent signals
3. Regulation of p53 stability
4. The dual model for p53 in maintaining genomic integrity
5. Regulation of the p53 tumor suppressor protein
6. Tumor suppressor p53: Regulation and function
7. p53: A transdominant regulator of transcription whose function is ablated by mutations occuring in human cancer
8. The DNA-binding domain of p53 contains the four conserved regions and the major mutation hot spots
9. p53 protein exhibits 3′-5′ exonuclease activity
10. A C-terminal α-helix plus basic region motif is the major structural determinant of p53 tetramerization
11. p53 domains: Identification and characterization of two autonomous DNA-binding regions
12. Nuclear accumulation of p53 protein is mediated by several nuclear localization signals and play a role in tumorigenesis
13. A leucine-rich nuclear export signal in the p53 tetramerization domain: Regulation of subcellular localization and p53 activity by NES masking
14. UV irradiation stimulates levels of p53 cellular tumor antigen in nontransformed mouse cells
15. A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in atalaxia telangiectasia
16. Regulation of p53 in response to DNA damage
17. Sequence-specific transcriptional activation is essential for growth suppression by p53
18. p53 transcriptional activity is essential for p53-dependent apoptosis following DNA damage
19. p53 mutations in human cancers
20. Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours
21. p53 and the Li-Fraumeni syndrome
22. DNA damage activates p53 through a phosphorylation-acetylation cascade
23. Covalent and noncovalent modifiers of the p53 protein
24. Regulation of p53 stability by mdm2
25. Definition of a consensus binding site for p53
26. A transcriptionally active DNA-binding site for human p53 protein complexes
27. SAGE transcript profiles for p53-dependent growth regulation
28. Identification and classification of p53-regulated genes
29. Analysis of p53-regulated gene expression patterns using oligonucleotide arrays
30. The mdm2 expression is induced by wild type p53 activity
31. The p53-mdm2 autoregulatory feedback loop
32. The mdm2 oncogene product forms a complex with the p53 protein and inhibits p53-mediated transactivation
33. Oncoprotein mdm2 conceals the activation domain of tumour suppressor p53
34. Regulation of transcription functions of the p53 tumor suppressor by the mdm2 oncogene
35. The mdm2 inhibits the G1 arrest and apoptosis functions of the p53 tumor suppressor protein
36. Cell type-specific inhibition of p53-mediated apoptosis by mdm2
37. In vivo ubiquitination and proteasome-mediated degradation of p53
38. The mdm2 promotes the rapid degradation of p53
39. Keeping an old friend under control: Regulation of p53 stability (1998)
40. Nucleo-cytoplasmic shuttling of the hdm2 oncoprotein regulates the levels of the p53 protein via a pathway used by the human immunodeficiency virus rev protein
41. The detection of environmental mutagens and potential carcinogens
42. Detection and classification of Mutagens: A set of base-specific Salmonella tester strains
43. SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity
44. Evaluation of the new system (umu-test) for the detection of environmental mutagens and carcinogens
45. New approaches to estimating the mutagenic potential of chemicals
46. A new approach to identifying genotoxic carcinogens: p53 induction as an indicator of genotoxic damage
47. Wild-type p53 mediates apoptosis by E1A, which is inhibited by E1B
48. Stabilization of the p53 tumor suppressor is induced by adenovirus 5 E1A and accompanies apoptosis
49. Accumulation of p53 induced by the adenovirus E1A protein requires regions involved in the stimulation of DNA synthesis
50. ARF tumor suppressor
51. Release of an inhibitor of angiogenese upon induction of wild type p53 expression in glioblastoma cells
52. Negative effects of wild-type and c-Myc on cellular growth and tumorigenicity of glioma cells. Implication of the tumor suppressor genes for gene therapy
53. The p53 protein is an unusually shaped tetramer that binds directly to DNA