Mode of DNA-protein interaction between the C-terminal domain of Escherichia coli RNA polymerase α subunit and T7 D promoter UP element

Nucleic Acids Research

Volumn 29, Issue 24, 2001, Pages 4909-4919

  Ozoline, Olga N ^a,b   Fujita, Nobuyuki ^a   Ishihama, Akira ^a  

^a NATIONAL INSTITUTE OF GENETICS   (Japan)

^b INSTITUTE OF CELL BIOPHYSICS   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; AMINO ACID; BACTERIAL DNA; DEOXYRIBONUCLEASE I; DNA BINDING PROTEIN; HYDROXYL GROUP; PROTEINASE; RNA POLYMERASE;

ALPHA CHAIN; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BACTERIOPHAGE T7; BACTERIUM MUTANT; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA HELIX; ESCHERICHIA COLI; GENE MAPPING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN DNA INTERACTION; RNA SYNTHESIS; RNA TRANSCRIPTION; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION INITIATION;

AMINO ACIDS; BACTERIOPHAGE T7; BASE SEQUENCE; BINDING SITES; DNA FINGERPRINTING; DNA, BACTERIAL; DNA, VIRAL; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MUTATION; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; REGULATORY SEQUENCES, NUCLEIC ACID; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0035945242     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. The functional and regulatory roles of sigma factors in transcription
2. Analysis of E. coli promoter sequences
3. Region 2.5 of the Escherichia coli RNA polymerase sigma 70 subunit is responsible for the recognition of the 'extended-10' motif at promoters
4. A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase
5. DNA-binding determinants of the alpha subunit of RNA polymerase: Novel DNA-binding domain architecture
6. Transcription factor recognition surface on the RNA polymerase alpha subunit is involved in contact with the DNA enhancer element
7. Structural organization of the RNA polymerase-promoter open complex
8. Interaction of the C-terminal domain of the E. coli RNA polymerase alpha subunit with the UP element: Recognizing the backbone structure in the minor groove surface
9. Fine structure of E. coli RNA polymerase-promoter interactions: Alpha subunit binding to the UP element minor groove
10. Bacterial promoter architecture: Subsite structure of UP elements and interactions with the carboxy-terminal domain of the RNA polymerase alpha subunit
11. Structure of open promoter complexes with Escherichia coli RNA polymerase as revealed by the DNase I footprinting technique: Compilation analysis
12. Escherichia coli promoters with UP elements of different strengths: Modular structure of bacterial promoters
13. Non-canonical sequence elements in the promoter structure. Cluster analysis of promoters recognized by Escherichia coli RNA polymerase
14. Transcription activation mediated by the carboxyl-terminal domain of the RNA polymerase alpha-subunit. Multipoint monitoring using a fluorescent probe
15. Rapid and efficient site-specific mutagenesis without phenotypic selection
16. Reconstitution of RNA polymerase
17. The two alpha subunits of Escherichia coli RNA polymerase are assymetrically arranged and contact different halves of the DNA upstream element
18. Synthesis of the protein cutting reagent iron (S)-1-(p-bromoacetamidobenzyl)ethylenediaminetetraacetate and conjugation to cystein side chains
19. Bipartite functional map of the E. coli RNA polymerase alpha subunit: Involvement of the C-terminal region in transcription activation by cAMP-CRP
20. Determination of the promoter strength in the mixed transcription system: Promoters of lactose, tryptophan and ribosomal protein L10 operons from Escherichia coli
21. Hydroxyl radical 'footprinting': High-resolution information about DNA-protein contacts and application to lambda repressor and Cro protein
22. Sequencing end-labeled DNA with base-specific chemical cleavages
23. Factor-independent activation of Escherichia coli rRNA transcription. II. Characterization of complexes of rrnB P1 promoters containing or lacking the upstream activator region with Escherichia coli RNA polymerase
24. Solution structure of the activator contact domain of the RNA polymerase alpha subunit
25. Interactions between the cyclic AMP receptor protein and the alpha subunit of RNA polymerase at the Escherichia coli galactose operon P1 promoter
26. Transcription activation at class I FNR-dependent promoters: Identification of the activating surface of FNR and the corresponding contact site in the C-terminal domain of the RNA polymerase alpha subunit
27. Transcription activation of class II CRP-dependent promoters: Identification of determinants in the C-terminal domain of the RNA polymerase alpha subunit
28. Protein-protein communication within the transcription apparatus
29. Promoter structure, promoter recognition, and transcription activation in prokaryotes
30. Mapping of the OxyR protein contact site in the C-terminal region of RNA polymerase alpha subunit
31. RNA polymerase alpha and sigma(70) subunits participate in transcription of the Escherichia coli uhpT promoter
32. Mutations in the alpha and sigma-70 subunits of RNA polymerase affect expression of the mer operon
33. Common fold in helix-hairpin-helix proteins
34. Crystal structure of the holliday junction DNA in complex with a single RuvA tetramer
35. Crystal structures of human DNA polymerase beta complexed with DNA: Implications for catalytic mechanism, processivity, and fidelity
36. Structural basis of DNA bridging by barrier-to-autointegration factor
37. The structure and the characteristic DNA binding property of the C-terminal domain of the RNA polymerase alpha subunit from Thermus thermophilus
38. Upstream interactions at the lambda pRM promoter are sequence nonspecific and activate the promoter to a lesser extent than an introduced UP element of an rRNA promoter
39. Use of monoacetyl-4-hydroxyaminoquinoline 1-Oxide to probe contacts between guanines and protein in the minor and major grooves of DNA. Interaction of Escherichia coli Integration Host Factor with its site in the early promoter of transposition enhancer of bacteriophage Mu
40. Monitoring of RNA polymerase-DNA UP element interaction by a fluorescent probe conjugated to alpha subunit