Conformationally restricted elongation factor G retains GTPase activity but is inactive in translocation on the ribosome

Molecular Cell

Volumn 6, Issue 2, 2000, Pages 501-505

  Peske, Frank ^a   Matassova, Natalia B ^a   Savelsbergh, Andreas ^a   Rodnina, Marina V ^a   Wintermeyer, Wolfgang ^a  

^a UNIVERSITY OF WITTEN HERDECKE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ELONGATION FACTOR G; GUANOSINE TRIPHOSPHATASE; TRANSFER RNA;

ARTICLE; BINDING KINETICS; CONTROLLED STUDY; CROSS LINKING; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROLYSIS; MOLECULAR DYNAMICS; MOLECULAR MODEL; NONHUMAN; PROTEIN CONFORMATION; PROTEIN DOMAIN; RIBOSOME; RNA TRANSPORT;

ESCHERICHIA COLI;

EID: 0033638447     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(00)00049-6     Document Type: Article

References (21)

1. Three-dimensional structure of the ribosomal translocase: Elongation factor G from Thermus thermophilus
2. Visualization of elongation factor G on the Escherichia coli 70S ribosome: The mechanism of translocation
3. EF-G-dependent GTP hydrolysis induces translocation accompanied by large conformational changes in the 70S ribosome
4. The structure of elongation factor G in complex with GDP: Conformational flexibility and nucleotide exchange
5. Equilibrium measurements of the interactions of guanine nucleotides with Escherichia coli elongation factor G and the ribosome
6. Truncated elongation factor G lacking the G domain promotes translocation of the 3' end but not of the anticodon domain of peptidyl-tRNA
7. Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase
8. Lock on/off disulfides identify the transmembrane signaling helix of the aspartate receptor
9. The conformational properties of elongation factor G and the mechanism of translocation
10. The crystal structure of elongation factor G complexed with GDP, at 2.7 Å resolution
11. Control of enzyme activity by an engineered disulfide bond
12. Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23S RNA
13. Hydrolysis of GTP by elongation factor G drives tRNA movement on the ribosome
14. Thiostrepton inhibits turnover but not GTP hydrolysis by elongation factor G on the ribosome
15. Role of domains 4 and 5 in elongation factor G functions on the ribosome
16. Large-scale movement of elongation factor G and extensive conformational changes of the ribosome during translocation
17. Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension
18. Conformational changes necessary for gene regulation by Tet repressor assayed by reversible disulfide bond formation
19. Mapping the position of translational elongation factor EF-G in the ribosome by directed hydroxyl radical probing
20. Translational elongation factor G: A GTP-driven motor of the ribosome
21. Model of maltose-binding protein/chemoreceptor complex supports intrasubunit signalling mechanism