Improving enzyme characteristics by gene shuffling; Application to β-glucosidase

Journal of Molecular Catalysis - B Enzymatic

Volumn 11, Issue 4-6, 2001, Pages 811-816

  Hayashi, Kiyoshi ^a   Ying, Li ^a   Singh, Satya ^a   Kaneko, Satoshi ^a   Nirasawa, Satoru ^a   Shimonishi, Tsuyoshi ^a   Kawata, Yasushi ^b   Imoto, Taiji ^c   Kitaoka, Motomitsu ^a  

^a NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

^b TOTTORI UNIVERSITY   (Japan)

^c KYUSHU UNIVERSITY   (Japan)

Author keywords

Glucosidase; Chimeric enzyme; Folding; Gene shuffling; GroEL ES

Indexed keywords

BETA GLUCOSIDASE; CHIMERIC PROTEIN;

AMINO ACID SEQUENCE; CELLVIBRIO; CELLVIBRIO GILVUS; CONFERENCE PAPER; DELETION MUTANT; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENETIC ENGINEERING; MUTANT; PROTEIN DOMAIN; PROTEIN FOLDING; RHIZOBIUM RADIOBACTER; THERMOSTABILITY;

EID: 0035931362     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1381-1177(00)00161-2     Document Type: Conference Paper

References (18)