Hydrophobic residues Phe552, Phe554, Ile562, Leu566, and Ile574 are required for oligomerization of anthrax protective antigen

Biochemical and Biophysical Research Communications

Volumn 287, Issue 2, 2001, Pages 542-549

  Ahuja, Nidhi ^a   Kumar, Praveen ^a   Bhatnagar, Rakesh ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

Alanine substitution; Anthrax protective antigen; Heptamers; Hydrophobic residues; Intoxication; Oligomerization; Site directed mutagenesis

Indexed keywords

ALANINE; ANTHRAX TOXIN; ANTHRAX VACCINE; BACTERIAL ANTIGEN; CELL SURFACE RECEPTOR; EDEMA FACTOR; ISOLEUCINE; LEUCINE; MUTANT PROTEIN; PHENYLALANINE; PROTECTIVE ANTIGEN; UNCLASSIFIED DRUG;

ACIDIFICATION; AMINO ACID SUBSTITUTION; ANIMAL CELL; ANTHRAX; ARTICLE; CHO CELL; CONTROLLED STUDY; CYTOSOL; ENDOCYTOSIS; ENDOSOME; HYDROPHOBICITY; INTERNALIZATION; LETHAL GENE; MOUSE; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; SITE DIRECTED MUTAGENESIS;

ANTHRAX;

EID: 0035929247     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5613     Document Type: Article

References (28)

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8. Purification of factor I and recognition of a third factor of anthrax toxin
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11. Inhibitors of receptor-mediated endocytosis block the entry of Bacillus anthracis adenylate cyclase toxin but not that of Bordetella pertussis adenylate cyclase toxin
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13. pH-dependent permeabilization of the plasma membrane of mammalian cells by anthrax protective antigen
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16. Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor
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18. Anthrax lethal factor cleaves the N-terminus of MAPKKs and induces tyrosine/threonine phosphorylation of MAPKs in cultured macrophages
19. Crystal structure of the anthrax toxin protective antigen
20. A deleted variant of B. anthracis protective antigen is non toxic and blocks anthrax toxin action in vivo
21. Trp346 and Leu352 residues in protective antigen are required for the expression of anthrax lethal toxin activity
22. The carboxyl-terminal end of protective antigen is required for receptor binding and anthrax toxin activity
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24. Point mutations in anthrax protective antigen that block translocation
25. 315, in anthrax toxin protective antigenis required for translocation of lethal factor
26. Cellular uptake of Clostridium botulinum C2 toxin requires oligomerization and acidification
27. Cellular uptake of Clostridium perfringens binary iota-toxin