Choline-binding domain as a novel affinity tag for purification of fusion proteins produced in Pichia pastoris

Biotechnology and Bioengineering

Volumn 74, Issue 2, 2001, Pages 164-171

  Caubin J ^a   Martin H ^a   Roa, A ^b   Cosano, I ^a   Pozuelo, M ^b   De La Fuente, J M ^b   Sanchez Puelles J M ^b   Molina, M ^a   Nombela, C ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Affinity tag; C LYTA; Heterologous expression; Yeast

Indexed keywords

AMINES; CHLORINE; ENZYMES; PURIFICATION;

CHLORINE-BINDING DOMAIN;

YEAST;

AMIDASE; BACTERIAL ENZYME; BINDING PROTEIN; CHOLINE; HYBRID PROTEIN; PROTEIN C LYTA; RECOMBINANT PROTEIN; TERTIARY AMINE; UNCLASSIFIED DRUG;

AFFINITY LABELING; ARTICLE; BINDING AFFINITY; BIOTECHNOLOGY; CARBOXY TERMINAL SEQUENCE; NONHUMAN; PICHIA PASTORIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; STREPTOCOCCUS PNEUMONIAE;

PICHIA PASTORIS; STREPTOCOCCUS PNEUMONIAE;

EID: 0035919870     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1106     Document Type: Article

References (20)

1. Design, total synthesis, and functional overexpression of the Candida rugosa lip1 gene coding for a major industrial lipase
2. Heterologous protein expression in the methylotrophic yeast Pichia pastoris
3. Pichia pastoris as a host system for transformations
4. The dacA gene of Bacillus stearother-mophilus coding for D-alanine carboxypeptidase: Cloning, structure and expression in Escherichia coli and Pichia pastoris
5. Chimeric phage-bacterial enzymes: A clue to the modular evolution of genes
6. Chimeric pneumococcal cell wall lytic enzymes reveal important physiological and evolutionary traits
7. Production of recombinant proteins by methylotrophic yeasts
8. Three dimensional structure of β-galactosidase
9. Structural characterization of the unligated and choline bound forms of the major pneumococcal autolysin LytA amidase
10. Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions
11. Novel method for detection of β-lactamases by using a chromogenic cephalosporin substrate
12. Single-step purification on DEAE-sephacel of recombinant polypeptides produced in E. coli
13. Contribution of novel choline-binding proteins to adherence, colonisation and immunogenicity of Streptococcus pneumoniae
14. Cloning and expression of gene fragments encoding the choline-binding domain of pneumococcal murein hydrolases
15. Immobilisation and single-step purification of fusion proteins using DEAE-cellulose
16. Structural requirements of choline derivatives for conversion of pneumococal amidase. A new single-step procedure for purification of this autolysin
17. High-frequency transformation of yeast: Autonomous replication of hybrid DNA molecules
18. The pUC plasmids, an M13 mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers