Purification of recombinant cutinase by extraction in an aqueous two-phase system facilitated by a fatty acid substrate

Biotechnology and Bioengineering

Volumn 73, Issue 6, 2001, Pages 465-475

  Fernandes, Sheryl ^a   Johansson, Göte ^a   Hatti Kaul, Rajni ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Aqueous two phase system; Butyrate; Cutinase; Extraction; Fluorescence spectroscopy; Interaction; Purification

Indexed keywords

ENZYMES; FATTY ACIDS; PURIFICATION;

TWO-PHASE SYSTEMS;

BIOTECHNOLOGY;

BUTYRIC ACID; CUTINASE; FATTY ACID;

ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SYNTHESIS; FLUORESCENCE SPECTROSCOPY; GEL PERMEATION CHROMATOGRAPHY; MICELLE; PARTITION COEFFICIENT; SACCHAROMYCES CEREVISIAE;

BIOCHEMISTRY; BUTYRATES; CARBOXYLIC ESTER HYDROLASES; CHROMATOGRAPHY, GEL; FATTY ACIDS; PHOSPHATES; POLYETHYLENE GLYCOLS; POTASSIUM COMPOUNDS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SPECTROMETRY, FLUORESCENCE;

EID: 0035919134     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1081     Document Type: Article

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