Denaturation of a recombinant cutinase from Fusarium solani in AOT-iso-octane reverse micelles: A steady-state fluorescence study

Photochemistry and Photobiology

Volumn 63, Issue 2, 1996, Pages 169-175

  Melo, E Pinho ^a   Costa, Silvia M B ^a   Cabral, Joaquim M S ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

FUSARIUM; FUSARIUM SOLANI;

EID: 0030038712     PISSN: 00318655     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1751-1097.1996.tb03009.x     Document Type: Article

References (33)

1. Dordick, J. S. (1989) Enzymatic catalysis in monophasic organic solvents. Enzyme Microb. Technol. 11, 194-211.
2. Luisi, P. L., M. Giomini, M. P. Pileni and B. H. Robinson (1988) Reverse micelles as hosts for proteins and small molecules. Biochim. Biophys. Acta 947, 209-246.
3. Castro, M. J. M. and J. M. S. Cabrai (1988) Reversed micelles in biotechnological processes. Biotechnol. Adv. 6, 151-167.
4. Martinek, K., N. L. Klyachko, A. V. Kabanov, Yu. L. Khmelnitsky and A. V. Levashov (1989) Micellar enzymology: its relation to membranology. Biochim. Biophys. Acta 981, 161-172.
5. Creagh, A. L., J. M. Prausnitz and H. W. Blanch (1993) Structural and catalytic properties of enzymes in reverse micelles. Enzyme Microb. Technol. 15, 383-392.
6. Grandi, C., R. E. Smith and P. L. Luisi (1981) Micellar solubilization of biopolymers in organic solvents. Activity and conformation of lysozyme in isooctane reverse micelles. J. Biol. Chem. 256, 837-843.
7. Desfosses, B., N. Cittanova, W. Urbach and M. Waks (1991) Ligand binding at membrane mimetic interfaces. Human serum albumin in reverse micelles. Eur. J. Biochem. 199, 79-87.
8. Steinmann, B., H. Jäckle and P. L. Luisi (1986) A comparative study of lysozyme conformation in various reverse micellar systems. Biopolymers 25, 1133-1156.
9. Walde, P., Q. Peng, N. W. Fadnavis, E. Battistel and P. L. Luisi (1988) Structure and activity of trypsin in reverse micelles. Eur. J. Biochem. 173, 401-409.
10. Brown, E. D., R. Y. Yada and A. G. Marangoni (1993) The dependence of the lipolytic activity of Rhizopus arrhizus lipase on surfactant concentration in aerosol-OT/isooctane reverse micelles and its relationship to enzyme structure. Biochim. Biophys. Acta 1161, 66-72.
11. Stamatis, H., A. Xenakis, M. Provelegiou and F. N. Kolisis (1993) Esterification reactions catalyzed by lipases in microemulsions: the role of enzyme localization in relation to its selectivity. Biotechnol. Bioeng. 42, 103-110.
12. Walde, P., D. Han and P. L. Luisi (1993) Spectroscopic and kinetic studies of lipases solubilized in reverse micelles. Biochemistry 32, 4029-4034.
13. Marangoni, A. G. (1993) Effects of the interaction of porcine pancreatic lipase with AOT/isooctane reverse micelles on enzyme structure and function follow predictable patterns. Enzyme Microb. Technol. 15, 944-949.
14. Melo, E. P., M. R. Aires-Barros and J. M. S. Cabral (1995) Triglycerides hydrolysis and stability of a recombinant cutinase from Fusarium solani in AOT-iso-octane reversed micelles. Appl. Biochem Biotechnol. 50, 45-56.
15. Sebastião, M. J., J M. S. Cabrai and M. R. Aires-Barros (1993) Synthesis of fatty acid esters by a recombinant cutinase in reversed micelles. Biotechnol. Bioeng. 42, 326-332.
16. Lauwereys, M., P. de Geus, J. de Meutter, P. Stanssens and G. Matthyssens (1993) Cloning, expression and characterization of cutinase, a fungal lipolytic enzyme. In Lipases - Structure, Mechanism and Genetic Engineering (Edited by L. Alberghina, R. D. Schmid and R. Verger), pp. 243-251. VCH, Weinheim, Germany.
17. Martinez, C., P. de Geus, M. Lauwereys, G. Matthyssens and C. Cambillau (1992) Fusarium solani cutinase is a lipolytic enzyme with a catalytic serine accessible to solvent. Nature 356, 615-618.
18. Menger, F M. and K. Yamada (1979) Enzyme catalysis in water pools. J. Am. Chem. Soc. 101, 6731-6734.
19. Melhuish, W. H. (1975) Modified technique for determining the wavelength-sensitivity curve of a spectrofluorimeter. Appl. Opt. 14, 26-27.
20. Medeiros, G. M. M., M. F. Leitão and S. M. B. Costa (1993) Fluorescence of acridine and acridine 9-carboxylic acid in anionic micelles. J. Photochem. Photobiol. A Chem. 72, 225-233.
21. Ferreira, L. F. V., S. M. B. Costa and E. J. Pereira (1991) Fluorescence quantum yield evaluation of strongly absorbing dye solutions as a function of the excitation wavelength. J. Photochem. Photobiol. A Chem. 55, 361-376.
22. Ricci, R. W. (1970) Deuten um-isotope effect on the fluorescence yields and lifetimes of indole derivatives including tryptophan and tryptamine. Photochem. Photobiol. 12, 67-75.
23. Henley, J. P. and A. Sadana (1985) Categorization of enzyme deactivations using a series-type mechanism. Enzyme Microb. Technol. 7, 50-60.
24. Longworth, J. W. (1971) Luminescence of polypeptides and proteins. In Excitated States of Proteins and Nucleic Acids (Edited by R. F. Steiner and I. Weinryb), pp. 319-484. Plenum Press, New York.
25. Ross, J. B. A., W. R. Laws, K. W. Rousslang and H. R. Wyssbrod (1992) Tyrosine fluorescence and phosphorescence from proteins and polypeptides. In Topics in Fluorescence Spectroscopy, Vol. 3, Biochemical Applications (Edited by J. R. Lakowicz), pp. 1-63. Plenum Press, New York.
26. Grinspan, H., J. Birnbaum and J. Feitelson (1966) Environmental effects on the ultraviolet absorption spectrum of tyrosine. Biochim. Biophys. Acta 126, 13-18.
27. Willis, K. J. and A. G. Szabo (1991) Fluorescence decay kinetics of tyrosinate and tyrosine hydrogen-bonded complexes. J. Phys. Chem. 95, 1585-1589.
28. Ragone, R., G. Colonna, C. Balestrieri, L. Servillo and G. Irace (1984) Determination of tyrosine exposure in proteins by second-derivative spectroscopy. Biochemistry 23, 1871-1875.
29. Cowgill, R. W. (1967) Fluorescence and protein structure. XI. Fluorescence quenching by disulfide and sulfhydryl groups. Biochim. Biophys. Acta 140, 37-44.
30. Weinryb, I. and R. F. Steiner (1971) The luminescence of the aromatic amino acids. In Excitated States of Proteins and Nucleic Acids (Edited by R. F. Steiner and I. Weinryb), pp. 277-318. Plenum Press, New York.
31. Genov, N., P. Nicolov, C. Betzel, K. Wilson and P. Dolashka (1993) Fluorescence properties of subtilisins and related proteinases (subtilases): relation to X-ray models. J. Photochem. Photobiol. B Biol. 18, 265-272.
32. Eicke, H. F. and J. Rehak (1976) On the formation of water/oil microemulsions. Helv. Chim. Acta 59, 2883-2891.
33. Vos, K., C. Laane, S. R. Weijers, A. van Hoek, C. Veeger and A. J. W. G. Visser (1987) Time resolved fluorescence and circular dichroism of porphyrin cytochrome c and Zn-porphyrin cytochrome c incorporated in reversed micelles. Eur. J. Biochem. 169, 259-268.