An analytical approach to the measurement of equilibrium binding constants: Application to EGF binding to EGF receptors in intact cells measured by flow cytometry

Biochemistry

Volumn 40, Issue 20, 2001, Pages 6142-6154

  Stein, Richard A ^a   Wilkinson, John C ^a   Guyer, Cheryl A ^a   Staros, James V ^a  

^a VANDERBILT UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; CELLS; CONCENTRATION (PROCESS); ISOTHERMS;

EQUILIBRIUM BINDING CONSTANTS;

BIOCHEMISTRY;

EPIDERMAL GROWTH FACTOR; EPIDERMAL GROWTH FACTOR RECEPTOR; LIGAND;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; CELL CULTURE; CELL DENSITY; CONTROLLED STUDY; FLOW CYTOMETRY; LIGAND BINDING; MATHEMATICAL ANALYSIS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR BINDING;

ANIMALS; BINDING, COMPETITIVE; CELL LINE; CHEMISTRY, ANALYTICAL; COMPUTER SIMULATION; EPIDERMAL GROWTH FACTOR; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; KINETICS; LIGANDS; MICE; MODELS, BIOLOGICAL; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECEPTOR, ERBB-2; TRANSFECTION;

EID: 0035918594     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi002817a     Document Type: Article

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