Design and production of genetically modified soybean protein with anti-hypertensive activity by incorporating potent analogue of ovokinin(2-7)

FEBS Letters

Volumn 497, Issue 1, 2001, Pages 50-54

  Matoba, Nobuyuki ^a   Doyama, Naomi ^a   Yamada, Yuko ^a   Maruyama, Nobuyuki ^a   Utsumi, Shigeru ^a   Yoshikawa, Masaaki ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Anti hypertensive peptide; Genetically modified food; Ovokinin; Soybean conglycinin; Spontaneously hypertensive rat

Indexed keywords

ARGINYLPROLYLLEUCYLLYSYLPROLYLTRYPTOPHAN; CHYMOTRYPSIN; KININ DERIVATIVE; OVOKININ; SOYBEAN PROTEIN; TRYPSIN; UNCLASSIFIED DRUG;

ALPHA CHAIN; AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; HYPERTENSION; ION EXCHANGE CHROMATOGRAPHY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN METABOLISM; SITE DIRECTED MUTAGENESIS;

ADMINISTRATION, ORAL; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; ANTIHYPERTENSIVE AGENTS; BLOOD PRESSURE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHYMOTRYPSIN; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG DESIGN; EGG PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLOBULINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; PRODRUGS; PROTEIN ENGINEERING; PROTEIN SUBUNITS; RATS; RATS, INBRED SHR; RECOMBINANT PROTEINS; SOYBEAN PROTEINS; SOYBEANS; TRYPSIN;

ESCHERICHIA COLI; GLYCINE MAX;

EID: 0035907064     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(01)02434-6     Document Type: Article

References (18)

1. Matoba N., Usui H., Fujita H., Yoshikawa M. FEBS Lett. 452:1999;181-184.
2. Matoba N., Yamada Y., Usui H., Nakagiri R., Yoshikawa M. Biosci. Biotechnol. Biochem. 65:2001;736-739.
3. Utsumi, S. (1992) in: Advances in Food and Nutrition Research (Kinsella, J.W., Ed.), Vol. 36, pp. 89-208, Academic Press, San Diego, CA.
4. Utsumi, S., Matsumura, Y. and Mori, T. (1997) in: Food Proteins and Their Applications (Damodaran, S. and Paraf, A., Eds.), pp. 257-291, Marcel Dekker, New York.
5. Maruyama N., Katsube T., Wada Y., Oh M.H., Barba de la Rosa A.P., Okuda E., Nakagawa S., Utsumi S. Eur. J. Biochem. 258:1998;854-862.
6. Sanger F., Nicklen S., Coulson A.R. Proc. Natl. Acad. Sci. USA. 74:1977;5463-5467.
7. Laemmli U.K. Nature. 227:1970;680-685.
8. Bradford M.M. Anal. Biochem. 72:1976;248-254.
9. Fujita H., Sasaki R., Yoshikawa M. Biosci. Biotechnol. Biochem. 59:1995;2344-2345.
10. Kim C.S., Kamiya S., Sato T., Utsumi S., Kito M. Protein Eng. 3:1990;725-731.
11. Katsube T., Kurisaka N., Ogawa M., Maruyama N., Ohtsuka R., Utsumi S., Takaiwa F. Plant Physiol. 120:1999;1063-1073.
12. Utsumi S., Kitagawa S., Katsube T., Higasa T., Kito M., Takaiwa F., Ishige T. Plant Sci. 102:1994;181-188.
13. Takaiwa F., Katsube T., Kitagawa S., Hisago T., Kito M., Utsumi S. Plant Sci. 111:1995;39-49.
14. Chua N.H., Sundaresan V. Curr. Opin. Biotechnol. 11:2000;117-119.
15. Ye X., Al-Babili S., Kloti A., Zhang J., Lucca P., Beyer P., Potrykus I. Science. 287:2000;303-305.
16. Goto F., Yoshihara T., Shigemoto N., Toki S., Takaiwa F. Nat. Biotechnol. 17:1999;282-286.
17. Walmsley A.M., Arntzen C.J. Curr. Opin. Biotechnol. 11:2000;126-129.
18. Chong D.K., Langridge W.H. Transgenic Res. 9:2000;71-78.