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Volumn 292, Issue 5520, 2001, Pages 1376-1378

A GDP/GTP exchange factor involved in linking a spatial landmark to cell polarity

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CYTOLOGY; ENZYMES; GROWTH KINETICS; POLARIZATION;

EID: 0035906952     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1060360     Document Type: Article
Times cited : (86)

References (33)
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    • For supplementary data, as well as more information about strains, plasmids, and methods, see Science Online (www.sciencemag.org/cgi/content/full/ 1060360/DC1).
  • 20
    • 0343908411 scopus 로고    scopus 로고
    • note
    • These cells showed ∼60% random budding (n = 200), as compared to 8% random budding of the wild-type strain (n = 200).
  • 21
    • 0342602607 scopus 로고    scopus 로고
    • note
    • a cells expressing Bud5-b1-GFP budded in an axial manner (85%; n = 200), whereas a/a cells expressing Bud5-b1-GFP budded in a random manner (93%; n = 200).
  • 22
    • 0343472665 scopus 로고    scopus 로고
    • note
    • Localization of Bud5-GFP in haploid bud8Δ and bud9Δ strains was indistinguishable from that in haploid wild-type cells.
  • 25
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    • T. Chen et al., Science 290, 1975 (2000).
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    • A. Fujita et al., Nature 372, 567 (1994).
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  • 27
    • 0342602601 scopus 로고    scopus 로고
    • note
    • In a similar approach, we were unable to detect coimmunoprecipitation of Bud5 and Bud3. Bud3 may not interact with Bud5 but rather may indirectly influence localization of Bud5. Alternatively, Bud5 may interact with Bud3 very transiently, which cannot be easily coimmunoprecipitated.
  • 28
    • 0343472660 scopus 로고    scopus 로고
    • note
    • Although a slightly less amount of Bud5 was coimmunoprecipitated or copurified with Axl2 from a/a cells compared to a cells, Axl2 was also recovered less efficiently from a/α cells by immunoprecipitation and GST column chromatography. We suspect that an unknown protein interacts with Axl2 in a/α cells, resulting in poor recovery of Axl2. One caveat of both approaches is that Axl2 is overexpressed when either a multicopy plasmid or the GAL promoter is used; hence it may not reflect the exact scenario occurring in the cell.
  • 32
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    • C. Geiger et al., EMBO J, 19, 2525 (2000).
    • (2000) EMBO J , vol.19 , pp. 2525
    • Geiger, C.1
  • 33
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    • note
    • We thank I. Herskowitz, A. Simcox, R. Hill, R. Tabtiang, E. Oakley, P. Wilson, and H. Chamberlin for comments on the manuscript; J. Pringle and B. Glick for discussions; and J. Pringle, M. Snyder, D. Kellogg, J. Chant, S. Sanders, and A. Straight for strains and plasmids. Supported by an NIH grant and a Basil O'Connor Starter Scholar Research Award from the March of Dimes Birth Defects Foundation to H.-O.P.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.