Formation of self-assembled, air-stable lipid bilayer membranes on solid supports

Langmuir

Volumn 17, Issue 8, 2001, Pages 2305-2307

  Ross, Eric E ^a   Bondurant, Bruce ^a   Spratt, Tony ^a   Conboy, John C ^a,b   O'Brien, David F ^a   Saavedra, S Scott ^a  

^a University of Arizona   (United States)

^b UNIVERSITY OF UTAH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LIPID BILAYER MEMBRANES; SOLID SUPPORTS;

ADSORPTION; CROSSLINKING; ORGANIC SOLVENTS; PHASE INTERFACES; POLYMERIC MEMBRANES; POLYMERIZATION; SELF ASSEMBLY; SURFACE ACTIVE AGENTS;

LIPIDS;

EID: 0035901804     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la0101752     Document Type: Article

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39. 3, saturated with Ar), without exposing the bilayer to air, incubated for 2 h, and then rinsed and dried. On the basis of LTV transmission measurements performed on four bilayers, a 2-h period was sufficient to achieve near quantitative polymerization.
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41. The acyl chains in a bis-SorbPC molecule are shorter by one bond than the acyl chains in a DOPC molecule. The thickness of a bis-SorbPC bilayer should therefore be slightly less thoan that of a DOPC bilayer, which has been determined to be about 45 Å. (Wiener, M. C.; White, S.H. Biophys. J. 1992, 61, 434.).
42. For comparison, the water contact angle measurements on a freshly cleaned Si wafer and on a Langmuir-Blodgett transferred monolayer of bis-SorbPC were <5° and 63 ± 5°, respectively. The contact angle for the bis-SorbPC monolayer is lower than that expected for a surface composed of saturated alkyl chains and reflects the presence of ester groups near the chain termini.
43. 28 is known to produce a higher degree of polymerization than direct UV irradiation of the sorbyl moiety. Since the polymerizable groups in bis-SorbPC are at the acyl chain termini, the inner and outer monolayers of a polymerized bis-SorbPC bilayer may be covalently linked. However, at this time we do not have any experimental evidence to support or refute this possibility.
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