Identification of essential active-site residues in ornithine decarboxylase of Nicotiana glutinosa decarboxylating both L-ornithine and L-lysine

Biochemical Journal

Volumn 360, Issue 3, 2001, Pages 657-665

  Lee, Y S ^a   Cho, Y D ^a  

^a Yonsei University   (South Korea)

Author keywords

Polyamine; Putrescine; Pyridoxal 5 phosphate (PLP); Site directed mutagenesis

Indexed keywords

BIOSYNTHESIS; CLONING; DEGRADATION; DNA; ENZYMES; ESCHERICHIA COLI; MUTAGENESIS;

CATALYTIC SITES;

BIOCHEMISTRY;

5,5' DITHIOBIS(2 NITROBENZOIC ACID); COMPLEMENTARY DNA; CYSTEINE; EFLORNITHINE; GLUTAMIC ACID; GLUTATHIONE TRANSFERASE; LYSINE; LYSINE DECARBOXYLASE; MUTANT PROTEIN; ORNITHINE; ORNITHINE DECARBOXYLASE; POLYAMINE; PROLINE; PUTRESCINE; PYRIDOXAL 5 PHOSPHATE; SERINE; THREONINE;

ARTICLE; BIOSYNTHESIS; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME PURIFICATION; ESCHERICHIA COLI; HUMAN; MOLECULAR MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; KINETICS; LYSINE; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; ORNITHINE; ORNITHINE DECARBOXYLASE; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TOBACCO;

DATURA; DATURA STRAMONIUM; ESCHERICHIA COLI; MAMMALIA; NICOTIANA; NICOTIANA GLUTINOSA; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0035893789     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3600657     Document Type: Article

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