RhoGDI-binding-defective mutant of Cdc42Hs targets to membranes and activates filopodia formation but does not cycle with the cytosol of mammalian cells

Biochemical Journal

Volumn 359, Issue 2, 2001, Pages 285-294

  Gibson, R M ^a   Wilson Delfosse, A L ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

GTPase; Prenylation; Targeting

Indexed keywords

BIOLOGICAL MEMBRANES; CELLS; CENTRIFUGATION; CHEMICAL ACTIVATION; IMMUNOLOGY; LIPIDS; MUTAGENESIS;

CYTOSOLS;

PROTEINS;

MUTANT PROTEIN; PROTEIN CDC42;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BINDING KINETICS; COMPLEX FORMATION; CONTROLLED STUDY; GENE TRANSLOCATION; GOLGI COMPLEX; IMMUNOFLUORESCENCE; NONHUMAN; POINT MUTATION; PRENYLATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; RAT;

ANIMALS; CDC42 GTP-BINDING PROTEIN; CELL LINE; CYTOSOL; GUANINE NUCLEOTIDE DISSOCIATION INHIBITORS; HUMANS; MICROSOMES; MODELS, BIOLOGICAL; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN ISOPRENYLATION; PSEUDOPODIA; RATS; RECOMBINANT PROTEINS; SUBCELLULAR FRACTIONS;

EID: 0035887166     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3590285     Document Type: Article

References (43)

1. The Ras-related protein Cdc42Hs and bradykinin promote formation of peripheral actin microspikes and filopodia in Swiss 3T3 fibroblasts
2. An essential role for Rho, Rac, and Cdc42 GTPases in cell cycle progression through G1
3. Cdc42 regulates anchorage-independent growth and is necessary for Ras transformation
4. Guanine nucleotide exchange factors regulate specificity of downstream signaling from Rac and Cdc42
5. Intracellular localization of the P21rho proteins
6. Rab GTPases: Master regulators of membrane trafficking
7. Rab GTPases in vesicular transport
8. Friends and family: The role of the Rab GTPases in vesicular traffic
9. Membrane targeting of the small GTPase Rab9 is accompanied by nucleotide exchange
10. Membrane association of Rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange
11. Ras genes
12. All ras proteins are polyisoprenylated but only some are palmitoyfated
13. Role of protein modification reactions in programming interactions between ras-related GTPases and cell membranes
14. Fsoprenoid modification of G25K (Gp), a low molecular mass GTP-binding protein distinct from p21ras
15. Post-translational modifications of p21rho proteins
16. Dissociation of LPA-induced cytoskeletal contraction from stress fiber formation by differential localization of RhoA
17. Translocation of activated Rho from the cytoplasm to membrane ruffling area, cell-cell adhesion sites and cleavage furrows
18. Spreading of differentiating human monocytes is associated with a major increase in membrane-bound CDC42
19. Integrin αllb β3-mediated translocation of CDC42Hs to the cytoskeleton in stimulated human platelets
20. Differential translocation of rho family GTPases by lysophosphatidic acid, endothelin-1, and platelet-derived growth factor
21. Subcellular localization of Cdc42p, a Saccharomyces cerevisiae GTP-binding protein involved in the control of cell polarity
22. Biologically active lipids are regulators of Rac.GDI complexation
23. Activation of NADPH oxidase involves the dissociation of p21rac from its inhibitory GDP/GTP exchange protein (rhoGDI) followed by its translocation to the plasma membrane
24. Translocation of Rac correlates with NADPH oxidase activation. Evidence for equimolar translocation of oxidase components
25. Guanine nucleotide exchange regulates membrane translocation of Rac/Rho GTP-binding proteins
26. Regulation mechanism of ERM (ezrin/radixin/moesin) protein/plasma membrane association: Possible involvement of phosphatidylinositol turnover and Rho-dependent signaling pathway
27. Newly synthesized Rho A, not Ras, is isoprenylated and translocated to membranes coincident with progression of the G1 to S phase of growth-stimulated rat FRTL-5 cells
28. Characterization of the interaction between RhoGDI and Cdc42Hs using fluorescence spectroscopy
29. Structure of the Rho family GTP-binding protein Cdc42 in complex with the multifunctional regulator RhoGDf
30. Kinetics of cdc42 membrane extraction by rho-GDI monitored by real-time fluorescence resonance energy transfer
31. Using PCR to engineer DNA
32. Association of Rab1B with GDP-dissociation inhibitor (GDI) is required for recycling but not initial membrane targeting of the Rab protein
33. Post-translational modification of low molecular mass GTP-binding proteins by isoprenoid
34. Rab8, a small GTPase involved in vesicular traffic between the TGN and the basolateral plasma membrane
35. Properties and kinetic mechanism of recombinant mammalian protein geranylgeranyltransferase type I
36. 4
37. Transient production of proteins using an adenovirus transformed cell line
38. Studies on a phytohemagglutinin from the lentil. IV. Direct binding studies of Lens culinaris hand myoglobin derivatives
39. Mammalian Cdc42 is a Brefeldin A-sensitive component of the Golgi apparatus
40. How RhoGDI binds Rho
41. Three-dimensional structure of an oncogene protein: Catalytic domain of human c-H-ras p21
42. Rab escort protein-1 is a multifunctional protein that accompanies newly prenylated rab proteins to their target membranes
43. The identification and characterization of a GDP-dissociation inhibitor (GDI) for the CDC42Hs protein