Protochlorophyllide oxidoreductase: A homology model examined by site-directed mutagenesis

Proteins: Structure, Function and Genetics

Volumn 44, Issue 3, 2001, Pages 329-335

  Townley, Helen E ^a   Sessions, Richard B ^a   Clarke, Anthony R ^a   Dafforn, Timothy R ^a   Trevor Griffiths, W ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

AM1; Chlorophyll; Dehydrogenases; Fluorescence; NADP; Photoenzyme; Protochlorophyll; Synechocystis

Indexed keywords

NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE; PROTOCHLOROPHYLLIDE;

ARTICLE; BINDING AFFINITY; BINDING SITE; CHEMICAL MODIFICATION; CYANOBACTERIUM; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; FLUORESCENCE; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; PROTEIN TERTIARY STRUCTURE; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; VALIDATION PROCESS;

AMINO ACID SEQUENCE; BINDING SITES; CYANOBACTERIA; FLUORESCENCE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NADP; OXIDOREDUCTASES; OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0035882551     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/prot.1098     Document Type: Article

References (26)

1. Protochlorophyllide photoreduction
2. Chlorophyll biosynthesis
3. Protochlorophyllide photoreduction
4. Purification and kinetic analysis of pea (Pisum sativum L) NADPH: Protochlorophyllide oxidoreductase expressed as a fusion with maltose-binding protein in Escherichia coli
5. A reappraisal of the mechanism of the photoenzyme protochlorophyllide reductase based on studies with the heterologously expressed protein
6. Protochlorophyllide oxidoreductase B-catalyzed protochlorophyllide photoreduction in vitro: Insight into the mechanism of chlorophyll formation in light-adapted plants
7. Protochlorophyllide reductase is homologous to human carbonyl reductase and pig 20-beta-hydroxysteroid dehydrogenase
8. A model of Plasmodium falciparum lactate dehydrogenase and its implications for the design of improved antimalarials and the enhanced detection of parasitaemia
9. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
10. Reconstitution of chlorophyllide formation by isolated etioplast membranes
11. Covalent labeling of the NADPH-protochlorophyllide oxidoreductase from etioplast membranes with N-phenylmaleimide
12. Cloning and sequencing of protochlorophyllide reductase
13. Structures important in mammalian 11 beta- and 17 beta-hydroxysteroid dehydrogenases
14. Participation of free radicals in photoreduction of protochlorophyllide to chlorophyllide in an artificial pigment-protein complex
15. Substrate specificity studies on protochlorophyllide reductase in barley (Hordeum vulgare) etioplast membranes
16. Pigment-free NADPH: Protochlorophyllide oxidoreductase from Avena sativa L-purification and substrate specificity
17. A single amino-acid substitution in lactate-dehydrogenase improves the catalytic efficiency with an alternative coenzyme
18. D175 discriminates between NADH and NADPH in the coenzyme binding-site of lactobacillus-delbrueckii subsp bulgaricus D-lactate dehydrogenase
19. Redesign of the coenzyme specificity of a dehydrogenase by protein engineering
20. A light-dependent complementation system for analysis of NADPH - Protochlorophyllide oxidoreductase - Identification and mutagenesis of 2 conserved residues that are essential for enzyme-activity
21. Identification of the chlB gene and the gene product essential for the light-independent chlorophyll biosynthesis in the cyanobacterium Plectonema boryanum
22. Genetic analysis of chlorophyll biosynthesis
23. A short-lived intermediate in the photoenzymatic reduction of protochlorophyllide into chlorophyllide at a physiological temperature
24. Synthesis and stereochemistry of hydroporphyrins