Phosphorylation of murine double minute clone 2 (MDM2) protein at serine-267 by protein kinase CK2 in vitro and in cultured cells

Biochemical Journal

Volumn 355, Issue 2, 2001, Pages 347-356

  Hjerrild, M ^a   Milne, D ^a   Dumaz, N ^a   Hay, T ^a   Issinger, O ^a   Meek, D ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

Oncogene; p53 pathway; Signalling

Indexed keywords

BIODEGRADATION; CELL CULTURE; CHROMATOGRAPHIC ANALYSIS; CONCENTRATION (PROCESS); FRACTIONATION; PROTEINS; SUBSTRATES;

PHOSPHORYLATION;

CLONING;

5,6 DICHLOROBENZIMIDAZOLE RIBOSIDE; CASEIN KINASE II; PROTEIN MDM2; SERINE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME SUBSTRATE; HUMAN; HUMAN CELL; IN VITRO STUDY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CASEIN KINASE II; CELL LINE; CELLS, CULTURED; DNA PRIMERS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NUCLEAR PROTEINS; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-MDM2; SERINE; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0035871222     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3550347     Document Type: Article

References (48)

1. The p53 pathway
2. Mechanisms of switching on p53: A role for covalent modification?
3. Analysis of p53-regulated gene expression patterns using oligonucleotide arrays
4. Regulation of p53 stability
5. Functions of the MDM2 oncoprotein
6. Oncoprotein MDM2 is a ubiquitin ligase E3 for tumour suppressor p53
7. Nuclear export is required for degradation of endogenous p53 by MDM2 and human papillomavirus E6
8. Mdm2 protein promotes rapid degradation of p53
9. Regulation of p53 stability by Mdm2
10. Nucleo-cytoplasmic shuttling of the hdm2 oncoprotein regulates the levels of the p53 protein via a pathway used by the human immunodeficiency virus rev protein
11. Analysis of the degradation function of Mdm2
12. Design of a synthetic Mdm2-binding mini protein that activates the p53 response in vivo
13. Regulation of p53 function and stability by phosphorylation
14. DNA damage-induced p53 stabilisation: No indication for an involvement of p53 phosphorylation
15. Rapid ATM-dependent phosphorylation of MDM2 precedes p53 accumulation in response to DNA damage
16. E1A signaling to p53 involves the p19ARF tumor suppressor
17. Multiple sites of in vivo phosphorylation in the MDM2 oncoprotein cluster within two important functional domains
18. SUMO-1 modification of Mdm2 prevents its self-ubiquitination and increases Mdm2 ability to ubiquitinate p53
19. The carboxy terminus of p53 mimics the polylysine effect of protein kinase CK2-catalysed MDM2 phosphorylation
20. Mdm2 phosphorylation by DNA-dependent protein kinase prevents interaction with p53
21. CK2: A protein kinase in need of control
22. Regulation of the specific DNA binding function of p53
23. Regulation of casein kinase II by growth factors: A reevaluation
24. Heat-induced relocalization of protein kinase CK2. Implication of CK2 in the context of cellular stress
25. Stress-induced activation of protein kinase CK2 by direct interaction with p38 mitogen-activated protein kinase
26. DNA damage triggers DRB-resistant phosphorylation of human p53 at the CK2 site
27. Functional activation of p53 via phosphorylation following DNA damage by UV but not gamma radiation
28. The p38MAPK inhibitor SB203580 alleviates ultraviolet-induced phosphorylation at Ser389 but not Ser15 and activation of p53
29. Identification of a CK2 phosphorylation site in mdm2
30. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product
31. p53-serine 15 phosphorylation stimulates transactivation function but does not directly influence interaction with HDM2
32. Rapid and efficient site-specific mutagenesis without phenotypic selection
33. Protein kinase CK2 structure-function relationship: Effects of the beta subunit on reconstitution and activity
34. Isolation and characterization of a monoclonal anti CK-2 alpha subunit antibody of the IgG1 subclass
35. Isolation and characterization of a monoclonal anti-protein kinase CK2 beta-subunit antibody of the IgG class for the direct detection of CK2 beta-subunit in tissue cultures of various mammalian species and human tumors
36. Analysis of multisite phosphorylation of the p53 tumour suppressor protein by tryptic phosphopeptide mapping
37. A structural basis for substrate specificities of protein ser/thr kinases: Primary sequence preference of casein kinases I and II, NIMA, phosphorylase kinase, calmodulin-dependent kinase II, CDK5, and Erk1
38. Development of inhibitors of protein kinases CK1 and CK2 and some related aspects, including donor and acceptor specificities and viral protein kinases
39. Phosphorylation of the p53 tumour-suppressor protein at three N-terminal sites by a novel casein kinase I-like enzyme
40. MDM2 is a target of simian virus 40 in cellular transformation and during lytic infection
41. The mdm-2 oncogene product forms a complex with the p53 protein and inhibits p53-mediated transactivation
42. Oncoprotein MDM2 conceals the activation domain of tumour suppressor p53
43. Association of p19ARF with Mdm2 inhibits ubiquitin ligase activity of Mdm2 for tumour suppressor p53
44. 32p orthophosphate in human fibroblasts
45. The p53 tumour suppressor protein is phosphorylated at Ser389 by casein kinase 2
46. Casein kinase II inhibits the renaturation of complementary DNA strands mediated by p53 protein
47. Phosphorylation of Ser392 stabilises the tetramer formation of tumor suppressor protein p53
48. Transcription factor E2F-1 is upregulated in response to DNA damage in a manner analogous to that of p53