Development of 16s rRNA targeted PCR methods for the detection and differentiation of Vibrio vulnificus in marine environments

Aquaculture

Volumn 193, Issue 3-4, 2001, Pages 199-211

  Kim, Myoung Sug ^a   Jeong, Hyun Do ^a  

^a Pukyong National University   (South Korea)

Author keywords

16S rRNA types; Combined primers; MPN (most probable numbers); PCR; Serotypes; V. vulnificus

Indexed keywords

BACTERIAL RNA; BACTERIUM DETECTION; BIOTYPE; DNA FRAGMENT; ENRICHMENT CULTURE; ESCHERICHIA COLI; MARINE ENVIRONMENT; MOST PROBABLE NUMBER; POLYMERASE CHAIN REACTION; RIBOSOME RNA; RNA 16S; STRAIN DIFFERENCE;

BACTERIUM; DETECTION METHOD; POLYMERASE CHAIN REACTION; RNA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; OSTREIDAE; VIBRIO VULNIFICUS; VIBRIONACEAE;

EID: 0035865941     PISSN: 00448486     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0044-8486(00)00495-6     Document Type: Article

References (30)

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2. Evidence that water transmits Vibrio vulnificus biotype 2 infections to eels
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4. Phenotypic characterization of Vibrio vulnificus biotype 2, a lipopolysaccharide-based homogeneous O serogroup within Vibrio vulnificus
5. Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells
6. Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification
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12. Polymerase chain reaction identification of Vibrio vulnificus in artificially contaminated oysters
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