Detection of angiotensin-converting enzyme insertion/deletion polymorphisms using real-time polymerase chain reaction and melting curve analysis with SYBR Green I on a GeneAmp 5700

Analytical Biochemistry

Volumn 289, Issue 2, 2001, Pages 300-303

  Hiratsuka, Masahiro ^a   Kishikawa, Yukinaga ^a   Narahara, Kaori ^a   Inoue, Tomoko ^a   Hamdy, Samar Ismail ^a   Agatsuma, Yasuyuki ^a   Tomioka, Yoshihisa ^a   Mizugaki, Michinao ^a  

^a TOHOKU UNIVERSITY HOSPITAL   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

ANGIOTENSIN-CONVERTING ENZYME; INSERTION/DELETION POLYMORPHISM; MELTING CURVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTIONS; SYBR GREEN I;

POLYMERASE CHAIN REACTION;

DIPEPTIDYL CARBOXYPEPTIDASE; DIPEPTIDYL CARBOXYPEPTIDASE INHIBITOR; FLUORESCENT DYE; GENE PRODUCT;

ALLELISM; ANALYTIC METHOD; ARTICLE; DIAGNOSTIC VALUE; DNA POLYMORPHISM; DRUG EFFICACY; DRUG RESPONSE; ENZYME ANALYSIS; ENZYME ASSAY; GENE DELETION; GENE INSERTION; GENETIC PREDISPOSITION; HEART LEFT VENTRICLE FAILURE; HUMAN; MELTING POINT; POLYMERASE CHAIN REACTION; PREDICTION; PRIORITY JOURNAL; RELIABILITY; RISK FACTOR; SEQUENCE ANALYSIS; TEMPERATURE DEPENDENCE; TREATMENT PLANNING;

EID: 0035865370     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4945     Document Type: Article

References (7)

1. An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels
2. Relation between changes in blood pressure and serum ACE activity after a single dose of enalapril and ACE genotype in healthy subjects
3. ACE (I/D) genotype as a predictor of the magnitude and duration of the response to an ACE inhibitor drug (enalaprilat) in humans
4. Effect of the insertion/deletion polymorphism of the angiotensin-converting enzyme gene on response to angiotensin-converting enzyme inhibitors in patients with heart failure
5. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization
6. The LightCycler: A microvolume multisample fluorimeter with rapid temperature control
7. PCR detection of the insertion/deletion polymorphism of the human angiotensin converting enzyme gene (DCP1) (dipeptidyl carboxypeptidase 1)