Use of a fluorescence microplate reader for the detection and characterization of metal-assisted peptide hydrolysis

Analytical Biochemistry

Volumn 289, Issue 2, 2001, Pages 196-201

  Grant, Kathryn B ^a   Pattabhi, Sowmya ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

Author keywords

Cleavage; Dipeptides; Fluorescamine; Palladium; Rates; Zinc

Indexed keywords

AMIDES; CURVE FITTING; FLUORESCENCE; HYDROLYSIS; METAL IONS; METALS; PALLADIUM; PEPTIDES; PLATES (STRUCTURAL COMPONENTS); RATE CONSTANTS; RATING; ZINC; ZINC COMPOUNDS;

CLEAVAGE; CONVENTIONAL APPROACH; DI-PEPTIDES; FLUORESCAMINES; FLUORESCENCE MICROPLATE READERS; MICROPLATE READERS; PEPTIDE HYDROLYSIS; STRUCTURAL STUDIES;

PALLADIUM COMPOUNDS;

FLUORESCAMINE; METAL ION; PALLADIUM; PEPTIDE; ZINC;

ARTICLE; CHEMICAL BOND; CHEMICAL REACTION KINETICS; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN STRUCTURE;

EID: 0035865324     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4963     Document Type: Article

References (31)

1. Nonenzymatic cleavage of peptide bonds: The methionine residues in bovine pancreatic ribonuclease
2. Specific chemical cleavage in high yield at the amino peptide bonds of cysteine and cystine residues
3. Specific cleavage of a protein by an attached iron chelate
4. Helix packing of lactose permease in Escherichia coli studied by site-directed chemical cleavage
5. 70 protein to the core subunits of Escherichia coli RNA polymerase, studied by iron-EDTA protein footprinting
6. Determinants of RNA polymerase a subunit for interaction with β, β′, and α subunits: Hydroxyl-radical protein footprinting
7. Conformation-dependent cleavage of staphylococcal nuclease with a disulfide-linked iron chelate
8. Vanadate-mediated photocleavage of rabbit skeletal myosin
9. A new strategy for selective protein cleavage
10. 2+ complex by hydrogen peroxide mediated protein cleavage
11. Chemistry and mechanism of vanadate-promoted photoxidative cleavage of myosin
12. Mapping of catalytic residues in the RNA polymerase active center
13. Mapping substrate-induced conformational changes in cAMP-dependent protein kinase by protein footprinting
14. Mapping cyclic nucleotide-induced conformational changes in cyclic AMP receptor protein by a protein footprinting technique using different chemical proteases
15. Direct measurement of the uncatalyzed rate of hydrolysis of a peptide bond
16. Rates of uncatalyzed peptide-bond hydrolysis in neutral solution and the transition-state affinities of proteases
17. Qualitative determination of N-terminal amino acids of peptides and proteins with cobalt(III) chelates
18. Toward artificial metallopeptidases: Mechanisms by which platinum(II) and palladium(II) complexes promote selective, fast hydrolysis of unactivated amide bonds in peptides
19. Cerium(IV)-cyclodextrin complex for peptide hydrolysis in neutral homogeneous solutions
20. 2 hydrolyzes both dipeptides and proteins
21. Specific cleavage of histidine-containing peptides by copper(II)
22. Peptide hydrolysis promoted by polynuclear and organometallic complexes of palladium(II) and platinum(II)
23. -1L]
24. Sequence-specific hydrolysis of peptides by metal-assisted autocatalysis of an internal hydroxy group
25. 2, a peptide modeling the potential metal binding site in the 'c-tail' region of histone H2A
26. Specific cleavage of immunoglobulin G by copper ions
27. Transition-metal complexes as alternatives to proteolytic enzymes. Regioselective cleavage of myoglobin by palladium(II) aqua complexes
28. Artificial metallopeptidases: Regioselective cleavage of lysozyme
29. Fluorescamine: A reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range
30. A fluorescence-based protein assay for use with a microplate reader
31. Amino acid analysis in the picomole range by precolumn derivatization and high-performance liquid chromatography