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Volumn 330, Issue 3, 2001, Pages 335-346
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Characterization of cytosolic sialidase from Chinese hamster ovary cells - Part I: Cloning and expression of soluble sialidase in Escherichia coli
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Author keywords
Affinity chromatography purification; GST sialidase; Neuraminidase; PGEX 2T plasmid vector
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Indexed keywords
4 METHYLUMBELLIFERYL ALPHA N ACETYLNEURAMINIC ACID;
COMPLEMENTARY DNA;
GLUTATHIONE TRANSFERASE;
HYBRID PROTEIN;
ISOPROPYL BETA THIOGALACTOPYRANOSIE;
N ACETYL 2,3 DIDEHYDRO 2 DEOXYNEURAMINIC ACID;
N ACETYLNEURAMINIC ACID DERIVATIVE;
PYRANOSIDE;
SIALIDASE;
SIALIDASE INHIBITOR;
UNCLASSIFIED DRUG;
AFFINITY CHROMATOGRAPHY;
ANIMAL CELL;
ARTICLE;
CHO CELL;
COMPETITIVE INHIBITION;
CONTROLLED STUDY;
ENZYME ANALYSIS;
ESCHERICHIA COLI;
MOLECULAR CLONING;
MOLECULAR WEIGHT;
NONHUMAN;
POLYACRYLAMIDE GEL ELECTROPHORESIS;
PRIORITY JOURNAL;
PROTEIN EXPRESSION;
ANIMALS;
CHO CELLS;
CHROMATOGRAPHY, AFFINITY;
CLONING, MOLECULAR;
CRICETINAE;
CYTOSOL;
DNA, COMPLEMENTARY;
ELECTROPHORESIS, AGAR GEL;
ENZYME INDUCTION;
ESCHERICHIA COLI;
GLUTATHIONE TRANSFERASE;
ISOPROPYL THIOGALACTOSIDE;
NEURAMINIDASE;
RECOMBINANT PROTEINS;
RESTRICTION MAPPING;
REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;
SUBSTRATE SPECIFICITY;
CRICETINAE;
CRICETULUS GRISEUS;
ESCHERICHIA COLI;
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EID: 0035864874
PISSN: 00086215
EISSN: None
Source Type: Journal
DOI: 10.1016/S0008-6215(00)00294-9 Document Type: Article |
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Times cited : (11)
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References (36)
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