A simple and rapid magnetic bead separation technique for the isolation of tetramer-positive virus-specific CD8 T cells

AIDS

Volumn 15, Issue 6, 2001, Pages 810-812

  McDermott, Adrian B ^a   Spiegel, Hans M L ^b   Irsch, Johannes ^c   Ogg, Graham S ^d   Nixon, Douglas F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CHILDREN'S NATIONAL MEDICAL CENTER   (United States)

^c MILTENYI BIOTEC GMBH   (Germany)

^d JOHN RADCLIFFE HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CD8 ANTIGEN; LEUKOCYTE ANTIGEN; PHYCOERYTHRIN; TETRAMER; VIRUS ANTIGEN;

ANTIGEN DETECTION; ARTICLE; ASSAY; CELL POPULATION; CELL SELECTION; CELL SEPARATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MAGNETISM; PRIORITY JOURNAL; SEROLOGY; T LYMPHOCYTE; TECHNIQUE; TISSUE CULTURE; VALIDATION PROCESS;

ANTIGENS, VIRAL; CD8-POSITIVE T-LYMPHOCYTES; CYTOMEGALOVIRUS; FLOW CYTOMETRY; HLA-A2 ANTIGEN; HUMANS; MAGNETICS; MICROSPHERES; PHOSPHOPROTEINS; SENSITIVITY AND SPECIFICITY; VIRAL MATRIX PROTEINS;

EID: 0035853395     PISSN: 02699370     EISSN: None     Source Type: Journal    
DOI: 10.1097/00002030-200104130-00024     Document Type: Article

References (9)

1. Characterization of circulating T cells specific for tumor-associated antigens in melanoma patients
2. Impaired function of circulating HIV-specific CD8(+) T cells in chronic human immunodeficiency virus infection
3. CD8+ T lymphocyte responses are induced during acute hepatitis C virus infection but are not sustained
4. HIV-specific CD8(+) T cells produce antiviral cytokines but are impaired in cytolytic function
5. Human immunodeficiency virus type 1- and cytomegalovirus-specific cytotoxic T lymphocytes can persist at high frequency for prolonged periods in the absence of circulating peripheral CD4(+) T cells
6. Direct isolation, phenotyping and cloning of low-frequency antigen-specific cytotoxic T lymphocytes from peripheral blood
7. Isolation of HIV-1-specific cytotoxic T lymphocytes using human leukocyte antigen-coated beads
8. Specificity of CTL interactions with peptide-MHC class I tetrameric complexes is temperature dependent
9. Characterization of the phytohemagglutinin-induced proliferating lymphocyte subpopulations in chronic lymphocytic leukemia patients using a clonogenic agar technique and monoclonal antibodies