NMR spectroscopic evidence for Mn2+(Mg2+) binding to a precursor-tRNA microhelix near the potential RNase P cleavage site

Journal of Molecular Biology

Volumn 305, Issue 2, 2001, Pages 181-189

  Zuleeg, Tilman ^a   Hartmann, Roland K ^b   Kreutzer, Roland ^c   Limmer, Stefan ^a,c  

^a UNIVERSITY OF BAYREUTH   (Germany)

^b UNIVERSITY OF LÜBECK   (Germany)

^c Ribopharma AG   (Germany)

Author keywords

Cleavage; Divalent metal ion; NMR; Precursor tRNA; RNase P

Indexed keywords

MAGNESIUM; MANGANESE; RIBONUCLEASE P; TRANSFER RNA;

ARTICLE; BINDING SITE; CATALYSIS; COMPLEX FORMATION; CONFORMATION; ENZYME MECHANISM; METAL BINDING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN DEGRADATION;

EID: 0035846957     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4299     Document Type: Article

References (31)

1. Divalent metal ion binding to a conserved wobble pair defining the upstream site of cleavage of group T self-splicing introns
2. Ribonuclease P
3. Coformational analysis of nucleic acids. Determination of backbone geometry of single-helical RNA and DNA in aqueous solution
4. Determination of metal ion binding sites within the hairpin ribozyme domains by NMR
5. Structure and thermodynamics of metal binding in the P5 helix of a group I intron ribozyme
6. The loop E-loop D region of Escherichia coli 5S rRNA: The solution structure reveals an unusual loop that may be important for binding ribosomal proteins
7. Solution structure of a substrate for the archaeal pre-tRNA splicing endonucleases: The bulge-helix-bulge motif
8. External guide sequences for an RNA enzyme
9. Ribonuclease P: Unity and diversity in a tRNA processing ribozyme
10. tRNA mimics
11. Solution structure and thermodynamics of a divalent metal ion binding site in an RNA pseudoknot
12. Nuclear magnetic resonance studies of the hammerhead ribozyme domain. Secondary structure formation and magnesium ion dependence
13. A tRNA circularization assay: Evidence for the variation of the conformation of the CCA end
14. Nucleic acid binding and catalysis by metal ions
15. The 3′-terminal end (NCCA) of tRNA determines the structure and stability of the aminoacyl acceptor stem
16. NMR evidence for helix geometry modifications induced by a G-U wobble base-pair in the acceptor arm of E. coli IRNAAla
17. Proton NMR studies of manganese ion binding to tRNA-derived acceptor arm duplexes
18. The chemical synthesis of biochemically active oligoribonucleotides using dimethylamino methylene protected purine H-phosphonates
19. 1H NMR spectroscopy
20. Important 2′-hydroxyl groups in model substrates for M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli
21. Pathway of activation by magnesium ions of substrates for the catalytic subunit of RNase P from Escherichia coli
22. Ala: Role of the G3 U70 base pair in synthetase recognition
23. Solution structure of cobalt(III)hexammine complexed to the GAAA tetraloop, and metal-ion binding to G. A mismatches
24. Catalytic RNA
25. Structural variation induced by different nucleotides at the cleavage site of the hammerhead ribozyme
26. Multiple magnesium ions in the ribonuclease P reaction mechanism
27. Conformation and dynamics of an RNA internal loop
28. Role of metal ions in the hydrolysis reaction catalyzed by RNase P RNA from Bacillus subtilis
29. Molecular interactions and metal binding in the theophylline-binding core of an RNA aptamer
30. tMet acceptor stem adopt unusual conformations