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Volumn 105, Issue 40, 2001, Pages 9887-9895

Spectral tuning of photoactive yellow protein. Theoretical and experimental analysis of medium effects on the absorption spectrum of the chromophore

Author keywords

[No Author keywords available]

Indexed keywords

PHOTOACTIVE YELLOW PROTEIN (PYP);

EID: 0035846381     PISSN: 10895647     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp011722v     Document Type: Article
Times cited : (52)

References (37)
  • 28
    • 0037596438 scopus 로고    scopus 로고
    • the position of Arg52 in solution differs significantly from that in the crystal. In addition, Genick et al. (ref 36 in this paper) stated that Arg52 is not required for charge stabilization of the chromophore. Irrespective of whether Arg52 is really the counterion or not, we think that it is of great importance to study how the electric field generated from protonated Arg52 influences the PYP shift.
    • In this study, according to the so-called electroneutrality rule and the crystal structure of PYP, protonated Arg52 was regarded as the counterion of the chromophore. On the other hand, there are reports implying that Arg52 does not work as counterion. According to the solution structure determined by NMR (Düx, P.; Rubinstenn, G.; Vuister, G.W.; Boelens, R.; Mulder, F.A.A.; Hård, K.; Hoff, W.D.; Kroon, A.R.; Crielaard, W.; Hellingwerf, K.J.; Kaptein, R. Biochemistry 1998, 37, 12689.), the position of Arg52 in solution differs significantly from that in the crystal. In addition, Genick et al. (ref 36 in this paper) stated that Arg52 is not required for charge stabilization of the chromophore. Irrespective of whether Arg52 is really the counterion or not, we think that it is of great importance to study how the electric field generated from protonated Arg52 influences the PYP shift.
    • (1998) Biochemistry , vol.37 , pp. 12689
    • Düx, P.1    Rubinstenn, G.2    Vuister, G.W.3    Boelens, R.4    Mulder, F.A.A.5    Hård, K.6    Hoff, W.D.7    Kroon, A.R.8    Crielaard, W.9    Hellingwerf, K.J.10    Kaptein, R.11
  • 34
    • 2242479732 scopus 로고    scopus 로고
    • To be published
    • To be published.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.