Probing the nature of the blue-shifted intermediate of photoactive yellow protein in solution by NMR: Hydrogen-deuterium exchange data and pH studies

Biochemistry

Volumn 39, Issue 47, 2000, Pages 14392-14399

  Craven, C J ^a,b   Derix, N M ^a   Hendriks, J ^c   Boelens, R ^a   Hellingwerf, K J ^c   Kaptein, R ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

^c UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; DEUTERIUM; HYDROGEN; PHOTOACTIVE YELLOW PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CATION EXCHANGE; CHEMICAL STRUCTURE; HALORHODOSPIRA HALOPHILA; HYDROPHOBICITY; NUCLEAR MAGNETIC RESONANCE; PH; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN STABILITY; RHODOSPIRILLUM; THERMODYNAMICS;

AMIDES; BACTERIAL PROTEINS; DEUTERIUM; HALORHODOSPIRA HALOPHILA; HYDROGEN-ION CONCENTRATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHOTORECEPTORS, MICROBIAL; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTONS; SOLUTIONS; THERMODYNAMICS; TITRIMETRY;

EID: 0034727659     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi001628p     Document Type: Article

References (29)

1. Properties of a water-soluble, yellow protein isolated from a halophilic phototrophic bacterium that has photochemical activity analogous to sensory rhodopsin
2. Structure of a protein photocycle intermediate by millisecond time-resolved crystallography
3. Protein folding thermodynamics applied to the photocycle of the photoactive yellow protein
4. Structural and dynamic changes of photoactive yellow protein during its photocycle in solution
5. trans/cis (Z/E) photoisomerization of the chromophore of photoactive yellow protein is not a prerequisite for the initiation of the photocycle of this photoreceptor protein
6. New insights into the photocycle of Ectothiorhodospira halophila photoactive yellow protein: Photorecovery of the long-lived photobleached intermediate in the Met100Ala mutant
7. Structure at 0.85 Å resolution of an early protein photocycle intermediate
8. Active site mutants implicate key residues for control of color and light cycle kinetics of photoactive yellow protein
9. Comparison of acid denaturation and light activation in the eubacterial blue-light receptor photoactive yellow protein
10. Global conformational changes upon receptor stimulation in photoactive yellow protein
11. Photoreaction cycle of photoactive yellow protein from Ectothiorhodospira halophila studied by low-temperature spectroscopy
12. Evidence for proton transfer from Glu-46 to the chromophore during the photocycle of photoactive yellow protein
13. Evidence for trans-cis isomerization of the p-coumaric acid chromophore as the photochemical basis of the photocycle of photoactive yellow protein
14. Concerted motions in the photoactive yellow protein
15. Glu46 donates a proton to the 4-hydroxycinnamate anion chromophore during the photocycle of photoactive yellow protein
16. Protonation deprotonation reactions triggered by photoactivation of photoactive yellow protein from Ectothiorhodospira halophila
17. Energy transduction on the nanosecond time scale: Early structural events in a xanthopsin photocycle
18. Photoactive yellow protein from the purple phototrophic bacterium, Ectothiorhodospira halophila: Quantum yield of photobleaching and effects of temperature, alcohols, glycerol, and sucrose on kinetics of photobleaching and recovery
19. NMR experiments for the study of photointermediates: Application to the photoactive yellow protein
20. Solution structure and backbone dynamics of the photoactive yellow protein
21. Primary structure effects on peptide group hydrogen exchange
22. Thermodynamic parameters from hydrogen exchange measurements
23. Solution structure of the POU-specific DNA-binding domain of Oct-1
24. 1.4 Angstrom structure of photoactive yellow protein, a cytosolic photoreceptor: Unusual fold, active-site, and chromophore
25. A residue-specific NMR view of the non-cooperative unfolding of a molten globule
26. An integrated kinetic analysis of intermediates and transition states in protein folding reactions