Mutational analysis of the genome-linked protein of cowpea mosaic virus

Virology

Volumn 290, Issue 1, 2001, Pages 21-29

  Carette, Jan E ^a   Kujawa, Alicja ^a   Gühl, Kerstin ^a   Verver, Jan ^a   Wellink, Joan ^a   Van Kammen, Ab ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

CPMV; Viral protein genome linked; Virus replication; VPg

Indexed keywords

AMINO ACID; PROTEIN VPG; SERINE; THREONINE; TYROSINE; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RNA;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; COWPEA MOSAIC COMOVIRUS; MOSAIC VIRUS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROTEIN INTERACTION; RNA REPLICATION; SITE DIRECTED MUTAGENESIS; VIRUS INFECTIVITY;

COMOVIRUS; COWPEA MOSAIC VIRUS;

EID: 0035841663     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.2001.1137     Document Type: Article

References (42)

1. Studies on hybrid comoviruses reveal the importance of three-dimensional structure for processing of the viral coat proteins and show that the specificity of cleavage is greater in trans than in cis
2. Association of cowpea mosaic virus-induced double-stranded RNA with a cytopathological structure in infected cells
3. Complete nucleotide sequence of bean pod mottle virus RNA1: Sequence comparisons and evolutionary relationships to other comoviruses
4. Tagging of plant potyvirus replication and movement by insertion of β-glucuronidase into the viral polyprotein
5. Cowpea mosaic virus RNA replication in crude membrane fractions from infected cowpea and Chenopodium armaranticolor
6. Improvements of the infectivity of in vitro transcripts from cloned cowpea mosaic virus cDNA: Impact of terminal nucleotide sequences
7. Evidence that the 32,000-Dalton protein encoded by bottom-component RNA of cowpea mosaic virus is a proteolytic processing enzyme
8. Expression of the bottom-component RNA of cowpea mosaic virus: Evidence that the 60-kilodalton VPg precursor is cleaved into single VPg and a 58-kilodalton polypeptide
9. Comoviruses: Molecular biology and replication
10. Cleavage site mutations in the encephalomyocarditis virus P3 region lethally abrogate the normal processing cascade
11. The genome-linked protein of cowpea mosaic virus is bound to the 5′ terminus of virus RNA by a phosphodiester linkage to serine
12. Construction of a "mutagenesis cartridge" for poliovirus genome-linked viral protein: Isolation and characterization of viable and nonviable mutants
13. Mutational analysis of the genome-linked protein VPg of poliovirus
14. Rapid and efficient site-specific mutagenesis without phenotypic selection
15. The nucleotide sequence of cowpea mosaic virus B RNA
16. The structure of cowpea mosaic virus replicative form RNA
17. Replacement of the tyrosine residue that links a potyviral VPg to the viral RNA is lethal
18. The VPg of tobacco etch virus RNA is the 49-kDa proteinase or the N-terminal 24-kDa part of the proteinase
19. A tyrosine residue in the small nuclear inclusion protein of tobacco vein mottling virus links the VPg to the viral RNA
20. Identification of an RNA hairpin in poliovirus RNA that serves as the primary template in the in vitro uridylylation of VPg
21. Protein-primed RNA synthesis by purified poliovirus RNA polymerase
22. The cowpea mosaic virus RNA 1-encoded 112 kDa protein may function as a VPg precursor in vivo
23. The NTP-binding motif in cowpea mosaic virus B polyprotein is essential for viral replication
24. Processing of VPg-containing polyproteins encoded by the B-RNA from cowpea mosaic virus
25. A regulatory role for the 32K protein in proteolytic processing of cowpea mosaic virus polyproteins
26. Primary structure and location of the genome-linked protein (VPg) of grapevine fanleaf nepovirus
27. Characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein VPg
28. Ultrastructural localization of nonstructural and coat proteins of 19 potyviruses using antisera to bacterially expressed proteins of plum pox potyvirus
29. O4-(5′-uridylyl) tyrosine is the bond between the genome-linked protein and the RNA of poliovirus
30. Protein-priming of DNA replication
31. Role of 3′-end sequences in infectivity of poliovirus transcripts made in vitro
32. Mutational evidence that the VPg is involved in the replication and not the movement of pea enation mosaic virus-1
33. RNA transcripts derived from a cloned full-length copy of the feline calicivirus genome do not require VpG for infectivity
34. Membrane-dependent uridylation of the genome-linked protein VPg of poliovirus
35. Synthesis of the complete 200K polyprotein encoded by cowpea mosaic virus B-RNA in insect cells
36. Protoplasts transiently expressing the 200K coding sequence of cowpea mosaic virus B-RNA support replication of M-RNA
37. 1H-NMR spectrum of the viral-protein-genome-linked polypeptide from cowpea mosaic virus
38. The structure of the covalent linkage between proteins and RNA in encephalomyocarditis virus
39. Infectious RNA transcripts derived from full-length DNA copies of the genomic RNAs of cowpea mosaic virus
40. Genetics of poliovirus
41. Complete protein linkage map of poliovirus P3 proteins: Interaction of polymerase 3Dpol with VPg and with genetic variants of 3AB
42. Chemical cleavage of 5′-linked protein from tobacco ringspot virus genomic RNAs and characterization of the protein-RNA linkage