Cloning of parsley flavone synthase I

Phytochemistry

Volumn 58, Issue 1, 2001, Pages 43-46

  Martens, Stefan ^a   Forkmann, Gert ^a   Matern, Ulrich ^b   Lukačin, Richard ^b  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

2 Oxoglutarate dependent dioxygenase; Apiaceae; Flavone synthase I cloning; Flavonoid biosynthesis; Heterologous expression; Petroselinum crispum

Indexed keywords

2 OXOGLUTARIC ACID; APIGENIN; COMPLEMENTARY DNA; FLAVONE DERIVATIVE; FLAVONE SYNTHASE I; NARINGENIN; OXYGENASE; RECOMBINANT PROTEIN; SYNTHETASE; UNCLASSIFIED DRUG;

ARTICLE; ASSAY; BIOSYNTHESIS; ENZYMOLOGY; GENE AMPLIFICATION; GENETICS; METABOLISM; MOLECULAR CLONING; NONHUMAN; PARSLEY; PLANT LEAF; PLANT SEED; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; YEAST CELL;

APIGENIN; CLONING, MOLECULAR; DNA, COMPLEMENTARY; FLAVANONES; FLAVONOIDS; MIXED FUNCTION OXYGENASES; PETROSELINUM; PLANT LEAVES; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

APIACEAE; PETROSELINUM CRISPUM;

EID: 0035839112     PISSN: 00319422     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0031-9422(01)00191-1     Document Type: Article

References (37)

1. Molecular cloning and biochemical characterization of novel cytochrome P450, flavone synthase II, that catalyzes direct conversion of flavanones to flavones
2. Novel flavonol 2-oxoglutarate-dependent dioxygenase: Affinity purification, characterization, and kinetic properties
3. The biosynthesis of penicillins and cephalosporins
4. 4-Hydroxyphenylpyruvate is an iron-tyrosinate protein
5. Conversion of flavanone to flavone, dihydroflavonol and flavonol with an enzyme system from cell cultures of parsley
6. Purification and characterization of flavone synthase I, a 2-oxoglutarate-dependent desaturase
7. Molecular cloning, sequence analysis, and in vitro expression of flavanone 3β-hydroxylase from Petunia hybrida
8. Molecular characterization of flavanone 3β-hydroxylase: Consensus sequence, comparison with related enzymes and the role of conserved histidines
9. Isolation and characterization of a 2-oxoglutarate-dependent dioxygenase involved in the second-to-last step in vindoline biosynthesis
10. 2-Oxoglutarate-dependent dioxygenase and related enzymes: Biochemical characterization
11. Anthocyanin biosynthesis in flowers of Matthiola incana. Flavanone 3- and flavonoid 3′-hydroxylases
12. Advances in flavonoid research since 1992
13. Hyoscyamine 6β-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, in alkaloid producing root cultures
14. Cofactor requirements for the soluble oxidases in the metabolism of the C20-gibberellins
15. Cloning and expression of flavonol synthase from Petunia hybrida
16. Protein hydroxylation: prolyl 4-hydroxylase, an enzyme with four cosubstrates and a multifunctional subunit
17. Induction and characterization of a NADPH-dependent flavone synthase from cell cultures of soybean
18. Heterologous expression and site directed mutagenesis of the 1-aminocyclopropane-1-carboxylate oxidase from kiwi fruit
19. Purification and properties of γ-butyrobetaine hydroxylase from Pseudomonas sp AK 1
20. Studies on the active site of deacetoxycephatosporin C synthase
21. Identification of strictly conserved histidine and arginine residues as part of the active site in Petunia hybrida flavanone 3β-hydroxylase
22. Purification of recombinant flavanone 3β-hydroxylase from Petunia hybrida and assignment of the primary site of proteolytic degradation
23. Site-directed mutagenesis of the active site serine 290 in flavanone 3β-hydroxylase from Petunia hybrida
24. The monomeric polypeptide comprises the functional flavanone 3β-hydroxylase from Petunia hybrida
25. The great diversity of reactions catalyzed by cytochromes P450
26. Genetic control of flavone synthase II activity in flowers of Gerbera hybrids
27. Cloning and expression of flavone synthase II from Gerbera hybrids
28. The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene
29. Molecular cloning of chick lysyl hydroxylase
30. Studies on two dioxygenases involved in the synthesis of tylosin in Streptomyces fradiae
31. A dilemma of dioxygenases (or where biochemistry and molecular biochemistry fail to meet)
32. Dioxygenases: Molecular structure and role in plant metabolism
33. Characterization of 4-hydroxyphenylpyruvate dioxygenase: Primary structure of the Pseudomonas enzyme
34. Purification and characterization of clavaminate synthase from Streptomyces clavuligerus an unusual oxidative enzyme in natural product biosynthesis
35. Hydroxylation of aspartic acid in domains homologous to the epidermal growth factor precursor is catalyzed by a 2-oxoglutarate-dependent dioxygenase
36. A new prolyl hydroxylase acting on poly-L-proline from suspension cultered cells of Vinca rosea
37. Expression, purification and characterization of 1-aminocyclopropane-1-carboxylate oxidase from tomato in Escherichia coli