Mutagenic Analysis of Functional Residues in Putative Substrate-binding Site and Acidic Domains of Vacuolar H+-Pyrophosphatase

Journal of Biological Chemistry

Volumn 276, Issue 10, 2001, Pages 7654-7660

  Nakanishi, Yoichi ^a   Saijo, Takanori ^a   Wada, Yoh ^b   Maeshima, Masayoshi ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; HYDROLYSIS; MAGNESIUM PRINTING PLATES; MUTAGENESIS; POSITIVE IONS; YEAST;

SUBSTRATE-BINDING FUNCTIONS;

ENZYMES;

ASPARTIC ACID; GLUTAMIC ACID; INORGANIC PYROPHOSPHATASE; LYSINE; MAGNESIUM ION; PHOSPHATE; PROTON PUMP; TRYPSIN; UNCLASSIFIED DRUG; VACUOLAR PROTON PYROPHOSPHATASE; ALANINE; AMINO ACID; COMPLEMENTARY DNA; MAGNESIUM; PROTON; VEGETABLE PROTEIN;

ACETABULARIA ACETABULUM; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARABIDOPSIS; ARTICLE; BINDING SITE; CATALYSIS; CELL VACUOLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; MUNG BEAN; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN MOTIF; RHODOSPIRILLUM RUBRUM; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; THERMOTOGA MARITIMA; BIOLOGICAL MODEL; CELL MEMBRANE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYMOLOGY; GENE LIBRARY; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; PLASMID; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN TERTIARY STRUCTURE; SEQUENCE HOMOLOGY;

ACETABULARIA; ACETABULARIA ACETABULUM; ARABIDOPSIS; PHASEOLUS (ANGIOSPERM); RHODOSPIRILLUM; RHODOSPIRILLUM RUBRUM; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; THERMOTOGA; THERMOTOGA MARITIMA; VIGNA RADIATA VAR. RADIATA;

ALANINE; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; AMINO ACIDS; BINDING SITES; CATALYTIC DOMAIN; CELL MEMBRANE; CONSERVED SEQUENCE; DNA MUTATIONAL ANALYSIS; DNA, COMPLEMENTARY; GENE LIBRARY; GLUTAMIC ACID; HYDROLYSIS; INORGANIC PYROPHOSPHATASE; LYSINE; MAGNESIUM; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLANT PROTEINS; PLASMIDS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; PROTONS; PYROPHOSPHATASES; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, AMINO ACID; TRYPSIN; VACUOLES;

EID: 0035831442     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M009743200     Document Type: Article

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