αArg-237 in Methylophilus methylotrophus (sp. W3A 1) Electron-transferring Flavoprotein Affords ∼200-Millivolt Stabilization of the FAD Anionic Semiquinone and a Kinetic Block on Full Reduction to the Dihydroquinone

Journal of Biological Chemistry

Volumn 276, Issue 23, 2001, Pages 20190-20196

  Talfournier, François ^a   Munro, Andrew W ^a,b   Basran, Jaswir ^a   Sutcliffell, Michael J ^a   Daff, Simon ^c   Chapman, Stephen K ^c   Scrutton, Nigel S ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME KINETICS; ENZYMES; NEGATIVE IONS; REDUCTION; THERMODYNAMICS; TITRATION;

ANAEROBIC REDOX TITRATION; ELECTRON-TRANSFERING FLAVOPROTEIN (ETF);

BIOCHEMISTRY;

ALANINE; ALPHA ARGININE; ANION; ARGININE; ARGININE DERIVATIVE; DIHYDROQUINONE; ELECTRON TRANSFERRING FLAVOPROTEIN; FLAVINE ADENINE NUCLEOTIDE; FLAVINE NUCLEOTIDE; FLAVOPROTEIN; GUANIDINE; HYDROQUINONE DERIVATIVE; MUTANT PROTEIN; OXIDOREDUCTASE; SEMIQUINONE; TRIMETHYLAMINE; TRIMETHYLAMINE DEHYDROGENASE; UNCLASSIFIED DRUG; 1,4 DIHYDROQUINONE; 1,4-DIHYDROQUINONE; BENZOQUINONE DERIVATIVE; PRIMER DNA; QUINONE DERIVATIVE; SEMIQUINONE RADICALS;

AMINO ACID SUBSTITUTION; ANAEROBIC METABOLISM; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ELECTRIC POTENTIAL; ELECTRON TRANSPORT; KINETICS; MEDICAL LITERATURE; METHYLOPHILUS METHYLOTROPHUS; MOLECULAR MODEL; NONHUMAN; OXIDATION; OXIDATION REDUCTION REACTION; POTENTIOMETRY; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN STABILITY; PROTEIN STRUCTURE; PUBLICATION; REDUCTION; SPECIES; THERMODYNAMICS; TITRIMETRY; WILD TYPE; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; NUCLEOTIDE SEQUENCE; SITE DIRECTED MUTAGENESIS;

METHYLOPHILUS METHYLOTROPHUS; NEGIBACTERIA;

ARGININE; BASE SEQUENCE; BENZOQUINONES; DNA PRIMERS; ELECTRON-TRANSFERRING FLAVOPROTEINS; FLAVOPROTEINS; KINETICS; METHYLOPHILUS METHYLOTROPHUS; MUTAGENESIS, SITE-DIRECTED; OXIDATION-REDUCTION; POTENTIOMETRY; QUINONES;

EID: 0035827531     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M010853200     Document Type: Article

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