The B12-binding subunit of glutamate mutase from Clostridium tetanomorphum traps the nucleotide moiety of coenzyme B12

Journal of Molecular Biology

Volumn 309, Issue 3, 2001, Pages 777-791

  Tollinger, Martin ^a,b   Eichmüller, Christian ^a,b   Konrat, Robert ^a,b   Huhta, Marja S ^a,b   Marsh, E Neil G ^a,b   Kräutler, Bernhard ^a,b  

^a UNIVERSITY OF INNSBRUCK   (Austria)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Coenzyme B12; Ligand binding; Nucleotide; Protein folding; Protein NMR spectroscopy

Indexed keywords

APOPROTEIN; COBALT; COBAMAMIDE; GLUTAMIC ACID; HISTIDINE; LIGAND; MUTASE; NITROGEN 15; NITROGEN DERIVATIVE; NUCLEOTIDE; NUCLEOTIDE BINDING PROTEIN; PROTEIN SUBUNIT;

ALPHA HELIX; ARTICLE; CLOSTRIDIUM; CONFORMATIONAL TRANSITION; ENZYME BINDING; LIGAND BINDING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN FOLDING; TIME;

CLOSTRIDIUM TETANOMORPHUM; MUS;

EID: 0035827176     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4696     Document Type: Article

References (41)

1. The glutamate mutase system. Assay and properties
2. Glutamate mutase
3. 12-dependent carbon-carbon and carbon-oxygen rearrangements
4. 12-binding domains of methionine synthase
5. Cloning and sequencing of glutamate mutase component S from Clostridium tetanomorphum
6. Adenosylcobalamin-dependent glutamate mutase: Examination of substrate and coenzyme binding in an engineering fusion protein possessing simplified subunit structure and kinetic properties
7. Adenosylcobalamin-dependent glutamate mutase from Clostridium tetanomorphum. Overexpression in Escherichia coli, purification, and characterization of the recombinant enzyme
8. 12-dependent glutamate mutase from Clostridium cochlearium produced in Escherichia coli
9. 12 radicals are generated: The crystal structure of methylmalonyl-coenzyme A mutase at 2 Å resolution
10. 12-dependent enzyme provides new mechanistic insights
11. 12-dependent enzymes
12. Binding of cob(II)alamin to the adenosylcobalamin-dependent ribonucleotide reductase from Lactobacillus leichmannii
13. 12-binding and the direct participation of a potassium ion in enzyme catalysis: X-ray structure of diol dehydratase
14. 12-binding subunit of glutamate mutase from C. tetanomorphum
15. 12-binding subunit of glutamate mutase from Clostridium cochlearium
16. 12: Experimente zur frage nach dem ursprung seiner molekularen struktur
17. Pure absorption gradient enhanced heteronuclear single quantum correlation spectroscopy with improved sensitivity
18. Localizing the NADP+ binding site on the MurB enzyme by NMR
19. Effects of chemical exchange on NMR spectra
20. 15N NMR relaxation
21. 12
22. 12 and related systems
23. 12 coenzyme: Methylcobalamin studies by X-ray and NMR methods
24. 12
25. 12-coenzymes containing benzimidazole or dimethylbenzimidazole
26. Structure of cobamide coenzymes: Influence of pH on absorption spectra and ionophoretic mobilities
27. Relationship between nuclear magnetic resonance chemical shift and protein secondary structure
28. The chemical shift index: A fast and simple method for the assignment of protein secondary structure through NMR spectroscopy
29. 2 (CPMG) methods
30. Structural and dynamic characterization of partially folded states of apomyoglobin and implications for protein folding
31. Thermolysis of the Co-C bond in adenosylcorrins. 3. Quantification of the axial base effect in adenosylcobalamin by the synthesis and thermolysis of axial base-free adenosylcobinamide. Insights into the energetics of enzyme-assisted cobalt-carbon bond homolysis
32. 12 electrochemistry and organometallic electrochemical synthesis
33. Solvent suppression with symmetrically-shifted pulses
34. Overcoming the overlap problem in the assignment of proton NMR spectra of larger proteins
35. 15N resonance assignments of the N-terminal SH3 domain of drk in folded and unfolded states using enhanced-sensitivity pulsed field gradient NMR techniques
36. Treatment of NOE constraints involving equivalent or nonstereoassigned protons in calculations of biomacromolecular structures
37. 1H-NMR
38. CHARMM: A program for macromolecular energy, minimization, and dynamics calculations
39. MOLMOL: A program for display and analysis of macromolecular structures