Catalysis of guanine nucleotide exchange on eIf2 by eIf2b: Can it be both a substituted enzyme and a sequential mechanism?

Biochemical and Biophysical Research Communications

Volumn 289, Issue 3, 2001, Pages 643-646

  Manchester, Keith L ^a  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

Author keywords

eIF2; eIF2B; Eukaryotic initiation; GDP; GDP dissociation stimulator protein; GTP; Guanine nucleotide exchange factor; Sequential enzyme mechanism; Substituted enzyme mechanism

Indexed keywords

GUANINE NUCLEOTIDE; GUANINE NUCLEOTIDE DISSOCIATION STIMULATOR PROTEIN; GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; INITIATION FACTOR; INITIATION FACTOR 2; UNCLASSIFIED DRUG;

CATALYSIS; DISSOCIATION; ENZYME MECHANISM; PRIORITY JOURNAL; PROTEIN SYNTHESIS; REACTION ANALYSIS; REVIEW;

EUKARYOTA;

EID: 0035824431     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.6010     Document Type: Review

References (18)

1. Initiation of protein synthesis in eukaryotic cells
2. Kinetic studies on the interactions of Escherichia coli K12 elongation factor Tu with GDP and elongation factor Ts
3. A study of the kinetic mechanism of elongation factor Ts
4. The kinetic mechanism of the release of nucleotide from elongation factor Tu promoted by elongation factor Ts determined by pressure relaxation studies
5. Characterization of the mammalian initiation factor eIF2B complex as a GDP dissociation stimulator protein
6. The catalytic mechanism of guanine nucleotide exchange factor action and competitive inhibition by phosphorylated eukaryotic initiation factor 2
7. Catalysis of guanine nucleotide exchange on eIF-2 by eIF-2B: Is it a sequential or substituted enzyme mechanism?
8. Mechanism of the nucleotide exchange reaction in eukaryotic polypeptide chain initiation. Characterization of the guanine nucleotide exchange factor as a GTP-binding protein
9. Purification and kinetic analysis of eIF2B from Saccharomyces cerevisiae
10. Purification, phosphorylation and control of the guanine-nucleotide-exchange factor from rabbit reticulocyte lysates
11. Studies on the role of eukaryotic nucleotide exchange factor in polypeptide chain initiation
12. 2- and guanine nucleotide exchange factor on the binding of guanine nucleotides to eukaryotic initiation factor 2
13. Mechanism of translational control by partial phosphorylation of the α-subunit of eukaryotic initiation factor 2
14. eIF-2B and the exchange of guanine nucleotides bound to eIF-2
15. Subunit assembly and guanine nucleotide exchange activity of eukaryotic initiation factor-2B expressed in Sf9 cells
16. eIF2 independently binds two distinct eIF2B sub-complexes that catalyze and regulate guanine-nucleotide exchange
17. Characterization of the initiation factor eIF2B and its regulation in Drosophila melanogaster
18. Photoaffinity labeling of the rabbit reticulocyte guanine nucleotide exchange factor and eukaryotic initiation factor 2 with 8-azidopurine nucleotides