Synthesis of the chelator lipid nitrilotriacetic acid ditetradecylamine (NTA-DTDA) and its use with the IAsys biosensor to study receptor-ligand interactions on model membranes

Biochimica et Biophysica Acta - Biomembranes

Volumn 1513, Issue 2, 2001, Pages 131-148

  Altin, Joseph G ^a   White, Felix A J ^a   Easton, Christopher J ^b  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^b AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

Chelator lipid; Erythropoietin; IAsys biosensor; Membrane; Nitrilotriacetic acid; Receptor ligand interaction

Indexed keywords

ACETIC ACID DERIVATIVE; DIMYRISTOYLPHOSPHATIDYLCHOLINE; ERYTHROPOIETIN RECEPTOR; HEXAHISTIDINE; HISTIDINE DERIVATIVE; LIPID; LIPOSOME; NITRILOTRIACETIC ACID DITETRADECYLAMINE; UNCLASSIFIED DRUG;

ARTICLE; ARTIFICIAL MEMBRANE; BINDING AFFINITY; BIOSENSOR; CONTROLLED STUDY; DENSITY; DISPERSION; FLUORESCENCE MICROSCOPY; HUMAN; KINETICS; LIPID MEMBRANE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN STABILITY; SYNTHESIS;

AMINES; BIOSENSING TECHNIQUES; CHELATING AGENTS; DIMYRISTOYLPHOSPHATIDYLCHOLINE; ERYTHROPOIETIN; LIGANDS; MEMBRANE PROTEINS; MEMBRANES, ARTIFICIAL; MICROSCOPY, FLUORESCENCE; MODELS, MOLECULAR; NITRILOTRIACETIC ACID; PROTEIN BINDING; RECEPTORS, ERYTHROPOIETIN;

MURINAE;

EID: 0035817263     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0005-2736(01)00344-3     Document Type: Article

References (38)

1. Two-dimensional crystallization of histidine-tagged, HIV-1 reverse transcriptase promoted by a novel nickel-chelating lipid
2. Electron crystallographic analysis of two-dimensional streptavidin crystals coordinated to metal-chelated lipid monolayers
3. Specific interaction and two-dimensional crystallization of histidine tagged yeast RNA polymerase I on nickel-chelating lipids
4. Two-dimensional crystallization on lipid layer: A successful approach for membrane proteins
5. Synthesis and characterisation of chelator-lipids for reversible immobilization of engineered proteins at self-assembled lipid interfaces
6. Molecular organization of histidine-tagged biomolecules at self-assembled lipid interfaces using a novel class of chelator lipids
7. Engineering protein-lipid interactions: Targeting of histidine-tagged proteins to metal-chelating lipid monolayers
8. Functional immobilization of a DNA-binding protein at a membrane interface via histidine tag and synthetic chelator lipids
9. Molecular recognition of histidine-tagged molecules by metal-chelating lipids monitored by fluorescence energy transfer and correlation spectroscopy
10. Orientation and two-dimensional organization of protein at chelator lipid interfaces
11. Metal-affinity separations: A new dimension in protein processing
12. Biosynthesis of glycosylphosphatidylinositol membrane anchors
13. Molecular recognition of antigen involves lattice formation between CD4, MHC class II and TCR molecules
14. Covalent dimerization of CD28/CTLA-4 and oligomerization of CD80/CD86 regulate T cell costimulatory interactions
15. CD4 dimerization and oligomerization: Implications for T-cell function and structure-based drug design
16. CD2 and the nature of protein interactions mediating cell-cell recognition
17. Making the T cell receptor go the distance: A topological view of T cell activation
18. T-cell activation: Interplay at the interface
19. Engrafting costimulator molecules onto tumor cell surfaces with chelator lipids: A potentially convenient approach in cancer vaccine development
20. Formation of supported planar bilayers by fusion of vesicles to supported phospholipid monolayers
21. Lipid and lipid-protein monolayers spread from a vesicle suspension: A microfluorescence film balance study
22. Phospholipid/alkanethiol bilayers for cell-surface receptor studies by surface plasmon resonance
23. Planar lipid bilayers on solid supports from liposomes-factors of importance for kinetics and stability
24. Surface plasmon resonance analysis at a supported lipid monolayer
25. Histidine-rich glycoprotein binds to human IgG and C1q and inhibits the formation of insoluble immune complexes
26. Differential binding of histidine-rich glycoprotein (HRG) to human IgG subclasses and IgG molecules containing k and l light chains
27. Reversible orientated surface immobilization of functional proteins on oxide surfaces
28. Design of supported membranes tethered via metal-affinity ligand-receptor pairs
29. Stable chelating linkage for reversible immobilization of oligohistidine tagged proteins in the BIAcore surface plasmon resonance detector
30. BIAcore analysis of histidine-tagged proteins using a chelating NTA sensor chip
31. Human erythropoietin receptor: Cloning, expression, and biologic characterization
32. Ligand binding properties of the human erythropoietin receptor extracellular domain expressed in Escherichia coli
33. The extracytoplasmic domain of the erythropoietin receptor forms monomeric complex with erythropoietin
34. Dimerization of the extracellular domain of the erythropoietin receptors by EPO: One high affinity and one low affinity interaction
35. Crystallographic evidence for preformed dimers of erythropoietin receptor before ligand activation
36. Production and ligand-binding characteristics of the soluble form of murine erythropoietin receptor
37. Ligand binding kinetics of a soluble full-length murine erythropoietin receptor
38. A novel system for convenient detection of low-affinity receptor-ligand interactions: Chelator-lipid liposomes engrafted with recombinant CD4 bind to cells expressing MHC class II