Phosphorylation causes subtle changes in solvent accessibility at the interdomain interface of methylesterase CheB

Journal of Molecular Biology

Volumn 307, Issue 4, 2001, Pages 967-976

  Hughes, Carrie A ^a   Mandell, Jeffrey G ^a   Anand, Ganesh S ^b,c   Stock, Ann M ^b,d,e   Komives, Elizabeth A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b RUTGERS UNIVERSITY   (United States)

^c UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY   (United States)

^d RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

^e HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Chemotaxis; H 2H exchange; MALDI TOF; Phosphoaspartate; Response regulator

Indexed keywords

ASPARTIC ACID; DEUTERIUM; ESTERASE; HYDROGEN; PROTEINE CHEB; SOLVENT; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME PHOSPHORYLATION; ENZYME STRUCTURE; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN DOMAIN;

EID: 0035815111     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4523     Document Type: Article

References (22)

1. Structural conservation in the CheY superfamily
2. The 1.9 Å resolution crystal structure of phosphono-CheY, an analog of the active form of the response regulator, CheY
3. NMR structure of activated CheY
4. Millisecond-timescale motions contribute to the function of the bacterial response regulator protein Spo0F
5. Phosphorylated aspartate in the structure of a response regulator protein
6. Conformational changes induced by phosphorylation of the FixJ receiver domain
7. Structure of a transiently phosphorylated switch in bacterial signal transduction
8. The two-component signaling pathway of bacterial chemotaxis: A molecular view of signal transduction by receptors, kinases, and adaptation enzymes
9. Multiple forms of the CheB methylesterase in bacterial chemosensing
10. Activation of methylesterase CheB: Evidence of a dual role for the regulatory domain
11. Structural basis for methylesterase CheB regulation by a phosphorylation-activated domain
12. Evidence for phosphorylation-dependent conformational changes in methylesterase CheB
13. Measurement of amide hydrogen exchange by MALDI-TOF mass spectrometry
14. Identification of protein-protein interfaces by decreased amide proton solvent accessibility
15. Crystal structure of the catalytic domain of the chemotaxis receptor methylesterase, CheB
16. Determinants of protein hydrogen exchange studied in equine cytochrome c
17. Activating and inhibitory mutations in the regulatory domain of CheB, the methylesterase in bacterial chemotaxis
18. 32P-phosphoramidate for use as a low molecular weight phosphodonor reagent
19. Divalent metal ion binding to the CheY protein and its significance to phosphotransfer in bacterial chemotaxis
20. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons
21. Determination of amide hydrogen exchange by mass spectrometry: A new tool for protein structure elucidation