Backbone dynamics of escherichia coli thioesterase/ protease i: Evidence of a flexible active-site environment for a serine protease

Journal of Molecular Biology

Volumn 307, Issue 4, 2001, Pages 1075-1090

  Huang, Yao Te ^a   Liaw, Yen Chywan ^b   Gorbatyuk, Vitaliy Ya ^a   Huang, Tai Huang ^a  

^a Institute of Biomedical Science   (Taiwan)

^b INSTITUTE OF MOLECULAR BIOLOGY   (Taiwan)

Author keywords

Enzyme catalysis; NMR relaxation; Protein dynamics; Serine protease; Thioesterase

Indexed keywords

BRINASE; HISTIDINE; SERINE; SERINE PROTEINASE; THIOL ESTER HYDROLASE;

ALPHA HELIX; ANISOTROPY; ARTICLE; CATALYSIS; CONTROLLED STUDY; ELECTRON SPIN RESONANCE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; ESCHERICHIA COLI; LIGAND BINDING; LIPOLYSIS; MOLECULAR DYNAMICS; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN MOTIF;

ESCHERICHIA COLI; MUS;

EID: 0035815106     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4539     Document Type: Article

References (67)

1. Dynamics of proteins: Elements and function
2. Characterizing global substates of myoglobin
3. The energy landscape in non-biological and biological molecules
4. Folding funnels, binding funnels, and protein function
5. Intrinsically unstructured proteins: Re-assessing the protein structure-function paradigm
6. Active site flexibility in enzyme catalysis
7. Stretching exercise-Flexibility in dihydrofolate reductase catalysis
8. NMR relaxation and protein mobility
9. Probing molecular motion by NMR
10. Protein dynamics from NMR
11. Protein dynamics from NMR
12. Properties of the thioesterase component obtained by limited trypsinization of the fatty acid synthetase complex
13. Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants
14. Microbial polyketide synthases: More and more prolific
15. Pseudomonas syningae phytotoxins: Mode of action, regulation, and biosynthesis by peptide and polyketide synthetases
16. Purification of long-chain acyl-CoA hydrolase from bovine heart microsomes and regulation of activity by lysophospholipids
17. Purification and properties of a palmitoyl-protein thioesterase that cleaves palmitate from H-ras
18. The human thioesterase II protein binds to a site on HIV-1 Nef critical for CD4 down-regulation
19. Betahydroxyldecanoyl thioesterase. I. Purification and properties
20. Studies on the mechanism of fatty acid synthesis. XIX. Preparation and general properties of palmityl thioesterase
21. Isolation and some properties of a proteolytic enzyme from Escherichia coli (Protease I)
22. Escherichia coli thioesterase I, molecular cloning and sequencing of the structural gene and identification as a periplasmic enzyme
23. Molecular cloning, sequencing, and mapping of the gene encoding protease I and characterization of protease and protease-defective Escherichia coli mutants
24. Thioesterases I and II of Escherichia coli. Hydrolysis of native acyl-acyl carrier protein thioesters
25. Protease I of Escherichia coli functions as a thioesterase in vivo
26. Defective export of a periplasmic enzyme disrupts regulation of fatty acid synthesis
27. The thioesterase I of Escherichia coli is an arylesterase and shows stereospecificity for protease substrates
28. A new family of lipolytic enzymes?
29. Multinuclear NMR resonance assignments and the secondary structure of Escherichia coli thioesterase/protease I: A member of a new subclass of lipolytic enzymes
30. The anatomy and taxonomy of protein structure
31. The α/β hydrolase fold
32. Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. I. Theory and range of validity
33. Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. I1. Analysis of experimental results
34. Deviation from the simple two-parameter model-free approach to the interpretation of nitrogen-15 nuclear magnetic relaxation of proteins
35. Backbone dynamics of Escherichia coli Ribonuclease HI: Correlation with structure and function in an active enzyme
36. Dynamics of the metallo-β-lactamase from Bacteroides fragilis in the presence and absence of a tight-binding inhibitor
37. Tryptophan in membrane proteins: Indole ring orientations and functional implications in the gramicidin channel
38. Protein structure by solid-state NMR
39. MOLMOL: A program for display and analysis of macromolecular structures
40. Application of a theory of enzyme specificity to protein synthesis
41. Enzymatic transition states and transition state analog design
42. Loop and subdomain movement in the mechanism of Escherichia coli dihydrofolate reductase: Crystallographic evidence
43. 1H NMR assignments of the Escherichia coli dihydrofolate reductase complex with folate: Evidence for a unique conformation of bound folate
44. Protein dynamics in enzymatic catalysis: Exploration of dihydrofolate reductase
45. Structural characterization of apofiavodoxin shows that the location of the stable nucleus differs among proteins with a flavodoxin-like topology
46. C-terminal his-tagging results in substrate specificity changes of the thioesterase I from E. coli
47. Calculation of protein extinction coefficients from amino acid sequence data
48. 2 values in proteins
49. 15N relaxation using inverse detected two-dimensional NMR spectroscopy: The central helix is flexible
50. 15N Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: Application to interleukin 1 beta
51. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions
52. 15N HSQC experiments optimized to retain full sensitivity
53. Spin-lattice relaxation measurements in slowly relaxing complex spectra
54. NHα) coupling constants in 15N-enriched proteins
55. Measurement of four-bond HN-H alpha J-couplings in staphylococcal nuclease
56. Chemical shifts as a tool for structural determination
57. 15N chemical shift referencing in biomolecular NMR
58. AURELIA, a program for computer-aided analysis of multidimensional NMR spectra
59. 13C nuclear spin relaxation
60. Long-range motional restrictions in a multidomain zinc-finger protein from anisotropic tumbling
61. 15N chemical shift anisotropy in Escherichia coli ribonuclease H in solution
62. Nuclear spin relaxation in ellipsoids undergoing rotational Brownian motion
63. 13C heteronuclear NMR spectroscopy
64. Backbone dynamics of proteins as studies by nitrogen-15 inverse detected heteronuclear NMR spectroscopy: Application to staphylococcal nuclease