Dominant-interfering Hsc70 mutants disrupt multiple stages of the clathrin-coated vesicle cycle in vivo

Journal of Cell Biology

Volumn 153, Issue 3, 2001, Pages 607-620

  Newmyer, Sherri L ^a   Schmid, Sandra L ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Clathrin coated vesicles; Endocytosis; Hsc70; Recycling; Transferrin receptor

Indexed keywords

ADENOSINE TRIPHOSPHATASE; CHAPERONE; CLATHRIN; COAT PROTEIN; HEAT SHOCK PROTEIN 70; TRANSCRIPTION FACTOR AP 1; TRANSCRIPTION FACTOR AP 2; TRANSFERRIN RECEPTOR; HEAT SHOCK COGNATE PROTEIN 70; HSPA8 PROTEIN, HUMAN;

ANIMAL CELL; ARTICLE; CELL SURFACE; CONTROLLED STUDY; ENDOCYTOSIS; GENE MUTATION; GENE OVEREXPRESSION; IN VIVO STUDY; INTERNALIZATION; MEMBRANE VESICLE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN FOLDING; RECYCLING; ADENOVIRUS; BIOLOGICAL MODEL; CELL FRACTIONATION; COATED VESICLE; CONFOCAL MICROSCOPY; GENETICS; HELA CELL; HUMAN; IMMUNOBLOTTING; KINETICS; METABOLISM; MUTATION; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; PROTEIN TRANSPORT; TIME;

ANIMALIA;

ADENOSINE TRIPHOSPHATASES; ADENOVIRIDAE; CELL FRACTIONATION; CLATHRIN; CLATHRIN-COATED VESICLES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOCYTOSIS; HELA CELLS; HSC70 HEAT-SHOCK PROTEINS; HSP70 HEAT-SHOCK PROTEINS; HUMANS; IMMUNOBLOTTING; KINETICS; MICROSCOPY, CONFOCAL; MODELS, BIOLOGICAL; MUTATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; RECEPTORS, TRANSFERRIN; TIME FACTORS;

EID: 0035809183     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.152.3.607     Document Type: Article

References (51)

1. Redundant and distinct functions for dynamin-1 and dynamin-2 isoforms
2. Aggregation of hsp70 and hsc70 in vivo is distinct and temperature-dependent and their chaperone function is directly related to non-aggregated forms
3. Microtubule and motor-dependent endocytic vesicle sorting in vitro
4. Auxilin-induced interaction of the molecular chaperone Hsc70 with clathrin baskets
5. Effect of nucleotides, peptides, and unfolded proteins on the self-association of the molecular chaperone HSC70
6. Stable clathrin: Uncoating protein (hsc70) complexes in intact neurons and their axonal transport
7. Binding of ATP and ATP analogues to the uncoating ATPase Hsc70 (70 kDa heat-shock cognate protein)
8. Induction of mutant dynamin specifically blocks endocytic coated vesicle formation
9. pH and the recycling of transferrin during receptor-mediated endocytosis
10. Association of the AP-3 adaptor complex with clathrin
11. The AP-3 complex required for endosomal synaptic vesicle biogenesis is associated with a casein kinase I alpha-like isoform
12. Similarity of the three-dimensional structures of actin and the ATPase fragment of a 70-kDa heat shock cognate protein
13. In polarized MDCK cells basolateral vesicles arise from clathrin-γ-adaptin-coated domains on endosomal tubules
14. The auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast
15. Effect of constitutive 70-kDa heat shock protein polymerization on its interaction with protein substrate
16. Dissociation of clathrin from coated vesicles by the uncoating ATPase
17. Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells
18. ATP- and cytosol-dependent release of adaptor proteins from clathrin-coated vesicles: A dual role for Hsc70
19. Construction of adenovirus vectors through Cre-lox recombination
20. In vivo expression of mammalian BiP ATPase mutants causes disruption of the endoplasmic reticulum
21. Mechanism of clathrin basket dissociation: Separate functions of protein domains of the DnaJ homologue auxilin
22. Receptor-mediated endocytosis is sensitive to antibodies against the uncoating ATPase (hsc70)
23. Hsc70 chaperones clathrin and primes it to interact with vesicle membranes
24. Mapping the role of active site residues for transducing an ATP-induced conformational change in the bovine 70-kDa heat shock cognate protein
25. Molecular chaperones: How J domains turn on Hsp70s
26. Adaptors for clathrin-mediated traffic
27. Clathrin
28. Hsp70 chaperone systems: Diversity of cellular functions and mechanism of action
29. mu1A-adaptin-deficient mice: Lethality, loss of AP-1 binding and rerouting of mannose 6-phosphate receptors
30. Stepwise assembly of a glucocorticoid receptor/hsp90 heterocomplex resolves two sequential ATP-dependent events involving first hsp70 and then hsp90 in opening of the steroid binding pocket
31. Endocytosis
32. Threonine 204 of the chaperone protein Hsc70 influences the structure of the active site, but is not essential for ATP hydrolysis
33. Lysine 71 of the chaperone protein Hsc70 is essential for ATP hydrolysis
34. Biosynthesis of the human transferrin receptor in cultured cells
35. The Saccharomyces cerevisiae APS1 gene encodes a homologue of the small subunit of the mammalian clathrin AP-1 complex: Evidence for functional interaction with clathrin at the Golgi complex
36. A yeast DNAJ protein required for uncoating of clathrin-coated vesicles in vivo
37. Complex formation between clathrin and uncoating ATPase
38. Characterization of D10S and K71E mutants of human cytosolic hsp70
39. Enzymatic recycling of clathrin from coated vesicles
40. An enzyme that removes clathrin coats: Purification of an uncoating ATPase
41. Clathrin-coated vesicle formation and protein sorting: An integrated process
42. Enzymatic dissociation of clathrin cages in a two-stage process
43. Dynamin: GTP controls the formation of constricted coated pits, the rate limiting step in clathrin-mediated endocytosis
44. A novel adaptor-related protein complex
45. Cytosol- and clathrin-dependent stimulation of endocytosis in vitro by purified adaptors
46. Clathrin, adaptors and eps15 in endosomes containing activated epidermal growth factor receptors
47. A novel class of clathrincoated vesicles budding from endosomes
48. Release of kinesin from vesicles by hsc70 and regulation of fast axonal transport
49. Identification of the universal cofactor (auxilin 2) in clathrin coat dissociation
50. Role of auxilin in uncoating clathrin-coated vesicles
51. Structural basis of the 70-kilodalton heat shock cognate protein ATP hydrolytic activity. I. Kinetic analyses of active site mutants