Effect of site-directed asparagine to isoleucine substitutions at the N-linked E1 glycosylation sites on rubella virus viability

Virus Research

Volumn 81, Issue 1-2, 2001, Pages 151-156

  Ramanujam, Meera ^a,c   Hofmann, Jorg ^b   Nakhasi, Hira L ^a   Atreya, Chintamani D ^a  

^a CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

^b Institute of Virology   (Germany)

^c Building 29A ^*  (United States)

Author keywords

Glycosylation; Mutations; Rubella virus; Structural proteins; Virus viability

Indexed keywords

ASPARAGINE; ISOLEUCINE; MEMBRANE PROTEIN; VIRUS RNA;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CELL LEVEL; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVE SITE; ENZYME GLYCOSYLATION; GENETIC TRANSFECTION; NONHUMAN; PRIORITY JOURNAL; RUBELLA; RUBELLA VIRUS; SITE DIRECTED MUTAGENESIS; VERO CELL; VIRION; VIRUS CAPSID; VIRUS MUTANT; VIRUS REPLICATION;

AMINO ACID SUBSTITUTION; ANIMALS; ASPARAGINE; CERCOPITHECUS AETHIOPS; CULTURE MEDIA, CONDITIONED; GLYCOSYLATION; IMMUNOBLOTTING; ISOLEUCINE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RUBELLA VIRUS; TRANSFECTION; VERO CELLS; VIRAL ENVELOPE PROTEINS; VIRION;

MIRIDAE; RUBELLA VIRUS;

EID: 0035807713     PISSN: 01681702     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1702(01)00374-4     Document Type: Article

References (27)

1. The rubella virus putative replicase interacts with the retinoblastoma tumor suppressor protein
2. Rubella virus maturation and production in two host cell systems
3. Calnexin: A membrane-bound chaperone of the endoplasmic reticulum
4. Molecular biology of rubella virus
5. Mutations in the retinoblastoma protein binding LXCXE motif of rubella virus putative replicase affect virus replication
6. Role of rubella virus glycoprotein domains in assembly of virus-like particles
7. Protein folding in the cell
8. Role of N-linked oligosaccharide recognition, glucose trimming, and calnexin in glycoprotein folding and quality control
9. Glucose trimming and reglucosylation determine glycoprotein association with calnexin in the endoplasmic reticulum
10. Using PCR to engineer DNA
11. Processing and intracellular transport of rubella virus structural proteins in COS cells
12. Analysis of rubella virus E1 glycosylation mutants expressed in COS cells
13. The role of glycosylation on hemagglutination and immunological reactivity of rubella virus
14. Low pH-induced confirmational changes of rubella virus envelop proteins
15. Polypeptide interactions with molecular chaperones and their relationship to in vivo folding
16. Analysis of the glycosylation sites of hepatitis C virus (HCV) glycoprotein E1 and the influence of E1 glycans on the formation of the HCV glycoprotein complex
17. Rubella virus glycoprotein interaction with the endoplasmic reticulum calreticulin and calnexin
18. Rubella virus 40S genome RNA specifies a 24S subgenomic mRNA that codes for a precursor to structural proteins
19. Growth restriction of dengue virus type 2 by site-specific mutagenesis of virus-encoded glycoproteins
20. Improvement of the specific infectivity of the rubella virus (RUB) infectious clone: Determinants of cytopathogenicity induced by RUB map to the nonstructural proteins
21. The influence of N-linked glycosylation on the antigenicity and immunogenicity of rubella virus E1 glycoprotein
22. Mutations in the E1 hydrophobic domain of rubella virus impair virus infectivity but not virus assembly
23. Interdependence of hemagglutinin glycosylation and neuraminidase as regulators of influenza virus growth: A study by reverse genetics
24. Construction of rubella virus genome-length cDNA clones and synthesis of infectious RNA transcripts
25. Effects of mutations in rubella virus E1 glycoprotein on E1-E2 interaction and membrane fusion activity
26. Nucleotide sequence of the 24S subgenomic messenger RNA of a vaccine strain (HPV77) of rubella virus: Comparison with wildtype strain (M33)
27. N-glycans of F protein differentially affect fusion activity of human respiratory syncytial virus