Mutations in the E1 hydrophobic domain of rubella virus impair virus infectivity but not virus assembly

Journal of Virology

Volumn 74, Issue 14, 2000, Pages 6637-6642

  Qiu, Zhiyong ^a,b   Yao, Jiansheng ^a   Cao, Hanwei ^a   Gillam, Shirley ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; CAPSID PROTEIN; GLYCINE; MEMBRANE PROTEIN; RNA; STRUCTURAL PROTEIN;

ARTICLE; MEMBRANE FUSION; NONHUMAN; PRIORITY JOURNAL; RUBELLA VIRUS; SEQUENCE ANALYSIS; VIRION; VIRUS ASSEMBLY; VIRUS INFECTIVITY; VIRUS MUTATION; VIRUS NUCLEOCAPSID;

AMINO ACID SUBSTITUTION; ANIMALS; CELL MEMBRANE; CERCOPITHECUS AETHIOPS; GIANT CELLS; MEMBRANE FUSION; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, TERTIARY; RUBELLA VIRUS; VERO CELLS; VIRAL ENVELOPE PROTEINS; VIRUS ASSEMBLY;

EID: 0033934282     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.14.6637-6642.2000     Document Type: Article

References (28)

1. Bardeletti, G., J. Tektoff, and G. Gautheron. 1979. Rubella virus maturation and production in two host cells systems. Intervirology 11:97-103.
2. Duffus, W. A., P. Levy-Mintz, M. R. Klimjack, and M. Kielian. 1995. Mutations in the putative fusion peptide of Semliki Forest virus affect spike protein oligomerization and virus assembly. J. Virol. 69:2471-2479.
3. Francki, R. I. B., C. M. Fauquet, D. L. Knudson, and F. Brown (ed.). 1991. Classification and nomenclature of viruses. Fifth report of the International Committee on Taxonomy of Viruses. Archives of Virology, suppl. 2. Springer-Verlag, Vienna, Austria.
4. Hobman, T. C., M. L. Lundslrom, and S. Gillam. 1990. Processing and transport of rubella virus structural proteins in COS cells. Virology 178:122-133.
5. Hobman, T. C., M. L. Lundstrom, C. A. Mauracher, L. Woodward, S. Gillam, and M. G. Farquhar. 1994. Assembly of rubella virus structural proteins into virus-like particles in transfected cells. Virology 202:575-585.
6. Katow, S., and A. Sugiura. 1988. Low pH-induced conformational changes of rubella virus envelope proteins. J. Gen. Virol. 69:2797-2807.
7. Kielian, M., M. R. Klimjack, S. Ghosh, and W. A. Duffus. 1996. Mechanisms of mutations inhibiting fusion and infection by Semliki Forest virus. J. Cell Biol. 134:865-872.
8. Kielian, M. C., and A. Helenius. 1986. Entry of alphaviruses, p. 91-119. In S. Schlesinger and M. J. Schlesinger (ed.). The Togaviridae and Flaviviridae. Plenum Publishing Corp., New York, N.Y.
9. Kobayashi, N. 1978. Hemolytic activity of rubella virus. Virology 89:6610-6612.
10. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
11. Lee, J. Y., D. Hwang, and S. Gillam. 1996. Dimerization of rubella virus capsid protein is not required for virus particle formation. Virology 216:223-227.
12. Levy-Mintz, P., and M. Kielian. 1991. Mutagenesis of the putative fusion domain of the Semliki Forest virus spike protein. J. Virol. 65:4292-4300.
13. Liebhaber, H. 1970. Measurement of rubella antibody by haemagglutination. I. Variables affecting rubella haemagglutination. J. Immunol. 104:818-825.
14. Liljestrom, P., and H. Garoff. 1991. A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Bio/Technology 9:1356-1361.
15. Mellman, I., R. Fuchs, and A. Helenius. 1986. Acidification of the endocytic and exocytic pathways. Annu. Rev. Biochem. 55:663-700.
16. Oker-Blom, C., N. Kalkkinen, L. Kaarianen, and R. F. Pettersson. 1983. Rubella virus contains one capsid protein and three envelope glycoproteins, E1, E2, E2a, and E2b. J. Virol. 46:964-973.
17. Oker-Blom, C., N. Kalkkinen, L. Kaarianen, and R. F. Pettersson. 1984. Rubella virus 40S RNA specifies a 24S subgenomic RNA that codes for a precursor to structural proteins. J. Virol. 49:403-408.
18. 2-C-E2-E1-COOH. J. Virol. 51:354-358.
19. Palmiter, R. D., R. R. Behringer, C. J. Quaife, F. Maxwell, I. H. Maxwell, and R. I. Brinster. 1987. Cell lineage ablation in transgenic mice by cell-specific expression of toxic gene. Cell 50:435-443.
20. Qiu, Z., D. Ou, H. Wu, T. C. Hobman, and S. Gillam. 1994. Expression and characterization of virus-like particles containing rubella virus structural proteins. J. Virol. 68:4086-4091.
21. Tarentino, A. L., and F. Maley. 1974. Purification and properties of an endo-β-N-acetylglucosaminidase from Streptomyces griseus. J. Biol. Chem. 249:811-817.
22. Towbin, H., Y. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
23. Vaananen, P., and L. Kaariaineen. 1980. Fusion and haemolysis of erythrocytes caused by three togaviruses: Semliki Forest, Sindbis and rubella. J. Gen. Virol. 46:467-475.
24. Waechter, D. E., and R. Baserga. 1982. Effect of methylation on expression of microinjected genes. Proc. Natl. Acad. Sci. USA 79:1106-1110.
25. Wahlberg, J. M., R. Bron, and H. Garoff. 1992. Membrane fusion of Semliki Forest virus involves homotrimers of the fusion protein. J. Virol. 66:7309-7318.
26. Wilson, C. A., J. W. Marsh, and M. W. Eiden. 1992. The requirements for viral entry differ from those for virally induced syncytium formation in NIH3T3/DTras cells exposed to Moloney murine leukemia virus. J. Virol. 66:7262-7269.
27. Yang, D., D. Hwang, Z. Qiu, and S. Gillam. 1998. Effects of mutations in rubella virus E1 glycoprotein on E1-E2 interaction and membrane fusion activity. J. Virol. 72:8747-8755.
28. Yao, J. S., and S. Gillam. 1999. Mutational analysis, using a full-length cDNA clone of rubella virus, of rubella virus E1 transmembrane and cytoplasmic domains required for virus release. J. Virol. 73:4622-4630.