A 'minimal' approach in design of flavivirus infectious DNA

Virus Research

Volumn 81, Issue 1-2, 2001, Pages 113-123

  Mishin, Vasiliy P ^a   Cominelli, Fabio ^a   Yamshchikov, Vladimir F ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

BGHTT; cDNA; CMV enhancer; Flavivirus; Infectious clone; Infectious DNA; Japanese encephalitis virus; Minimal CMV promoter

Indexed keywords

COMPLEMENTARY DNA; PLASMID DNA; VIRUS DNA; VIRUS PROTEIN; VIRUS RNA;

5' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; CELL STRAIN BHK; CLONE; CONTROLLED STUDY; CYTOMEGALOVIRUS; DNA MODIFICATION; DNA TRANSCRIPTION; DNA TRANSFECTION; ESCHERICHIA COLI; GENE CASSETTE; GENE CONSTRUCT; GENE EXPRESSION; GENE INSERTION; GENETIC STABILITY; HOST; JAPANESE ENCEPHALITIS VIRUS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; VERO CELL; VIROGENESIS; VIRUS INFECTION;

5' UNTRANSLATED REGIONS; CELL LINE; CLONING, MOLECULAR; CYTOMEGALOVIRUS; DNA, COMPLEMENTARY; DNA, VIRAL; ENCEPHALITIS VIRUS, JAPANESE; ENHANCER ELEMENTS (GENETICS); ESCHERICHIA COLI; PLASMIDS; PROMOTER REGIONS (GENETICS); RNA, VIRAL; TRANSCRIPTION, GENETIC; TRANSFECTION;

BACTERIA (MICROORGANISMS); CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS; ESCHERICHIA COLI; EUKARYOTA; FLAVIVIRUS; JAPANESE ENCEPHALITIS FLAVIVIRUS; JAPANESE ENCEPHALITIS VIRUS; RNA VIRUSES;

EID: 0035807708     PISSN: 01681702     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1702(01)00371-9     Document Type: Article

References (35)

1. From the cover: Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome
2. Eukaryotic promoters drive gene expression in Escherichia coli
3. Development of DNA vaccines for foot-and-mouth disease, evaluation of vaccines encoding replicating and non-replicating nucleic acids in swine
4. Infectious transcripts and cDNA clones of RNA viruses
5. Sindbis virus expression vectors: Packaging of RNA replicons by using defective helper RNAs
6. Sequence and secondary structure analysis of the 5′-terminal region of flavivirus genome RNA
7. Infectious cDNA clones of langat tick-borne flavivirus that differ from their parent in peripheral neurovirulence
8. Flavivirus genome organization, expression, and replication
9. Transfer and expression of plasmids containing human cytomegalovirus immediate-early gene 1 promoter-enhancer sequences in eukaryotic and prokaryotic cells
10. Powerful and versatile enhancer-promoter unit for mammalian expression vectors
11. Infectious transcripts of tick-borne encephalitis virus, generated in days by RT-PCR
12. A plasmid-based self-amplifying Sindbis virus vector
13. Intron insertion facilitates amplification of cloned virus cDNA in Escherichia coli while biological activity is reestablished after transcription in vivo
14. An enhancer in the first intron of the human purine nucleoside phosphorylase-encoding gene
15. Synthesis and characterization of an infectious dengue virus type-2 RNA genome (New Guinea C strain)
16. Phylogeny of the genus Flavivirus
17. Construction of a stable and highly infectious intron-containing cDNA clone of plum pox potyvirus and its use to infect plants by particle bombardment
18. An artificial enhancer with multiple response elements stimulates prokaryotic transcriptional activation medicated by various regulatory proteins
19. Nucleotide sequence of the virulent SA-14 strain of Japanese encephalitis virus and its attenuated vaccine derivative, SA-14-14-2
20. Nucleotide sequence and infectious cDNA clone of the L1 isolate of Pea seed-borne mosaic potyvirus
21. Infectious RNA transcripts from full-length dengue virus type 2 cDNA clones made in yeast
22. In vitro processing of dengue virus type 2 nonstructural proteins NS2A, NS2B, and NS3
23. Cloned poliovirus complementary DNA is infectious in mammalian cells
24. Flaviviridae: The viruses and their replication
25. Transcription of infectious yellow fever RNA from full-length cDNA templates produced by in vitro ligation
26. Modulations of the in vitro translational efficiencies of Yellow Fever virus mRNAs: Interactions between coding and noncoding regions
27. Assaying the polyadenylation state of mRNAs
28. Alphavirus vectors for gene expression and vaccines
29. Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals
30. Infectious Japanese encephalitis virus RNA can be synthesized from in vitro-ligated cDNA templates
31. Complete nucleotide sequence of the Japanese encephalitis virus genome RNA
32. Promoter-regulatory region of the major immediate early gene of human cytomegalovirus
33. Stable high-level expression of heterologous genes in vitro and in vivo by noncytopathic DNA-based kunjin virus replicon vectors
34. A new strategy in design of +RNA virus infectious clones enabling their stable propagation in E. coli