The effect of methotrexate on actin and invasiveness of hepatoma Morris 5123 cells in ulture

Acta Biochimica Polonica

Volumn 48, Issue 4, 2001, Pages 1051-1060

  Otrocka, Magdalena ^a   Verschueren, Hendrik ^b   Malicka Błaszkiewicz, Maria ^a  

^a UNIVERSITY OF WROC AW   (Poland)

^b PASTEUR INSTITUTE OF BRUSSELS   (Belgium)

Author keywords

Actin; Hepatoma Morris 5123; Invasiveness; Methotrexate

Indexed keywords

ACTIN; ANTINEOPLASTIC ANTIMETABOLITE; BIOMATERIAL; COLLAGEN; LAMININ; MATRIGEL; METHOTREXATE; PROTEOGLYCAN;

ANIMAL; ARTICLE; CANCER INVASION; CELL CULTURE; CELL MOTION; CHEMISTRY; CYTOPLASM; CYTOSOL; DOSE RESPONSE; DRUG COMBINATION; FLUORESCENCE MICROSCOPY; LIVER CELL CARCINOMA; METABOLISM; RAT;

ACTINS; ANIMALS; ANTIMETABOLITES, ANTINEOPLASTIC; BIOCOMPATIBLE MATERIALS; CARCINOMA, HEPATOCELLULAR; CELL MOVEMENT; COLLAGEN; CYTOPLASM; CYTOSOL; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG COMBINATIONS; LAMININ; METHOTREXATE; MICROSCOPY, FLUORESCENCE; NEOPLASM INVASIVENESS; PROTEOGLYCANS; RATS; TUMOR CELLS, CULTURED;

EID: 0035755699     PISSN: 0001527X     EISSN: None     Source Type: Journal    
DOI: 10.18388/abp.2001_3866     Document Type: Article

References (35)

1. Janmey, P.A. & Chaponnier, C. (1995) Medical aspects of the actin cytoskeleton. Curr. Opin. Cell Biol. 7, 111-117.
2. Sheterline, P., Clayton, J. & Sparrow, J.C. (1998) Protein Profile. Actin, 4th edn. pp. 1-13, Oxford University Press, New York.
3. Ben-Ze'ev, A. (1985) The cytoskeleton in cancer cells. Biochim. Biophys. Acta 780, 197-212.
4. Hansell, E.J., Frisch, S.M., Tremble, P., Murnane, J.P. & Werb, Z. (1995) Simian virus 40 transformation alters the actin cytoskeleton, expression of matrix metalloproteinases and inhibitors of metalloproteinases, and invasive behavior of normal and ataxia-telagiectasia human skin fibroblasts. Biochem. Cell Biol. 73, 737-389.
5. Button, E., Shapland, C. & Lawson, D. (1995) Actin, its associated proteins and metastasis. Cell Motil. Cytoskel. 30, 247-251.
6. Pokorna, E., Jordan, P.W., O'Neill, C.H., Zicha, D., Gilbert, C.S. & Vesely, P. (1994) Actin cytoskeleton and motility in rat sarcoma cell populations with different metastatic potential. Cell Motil. Cytoskel. 28, 25-33.
7. Le, P.U., Nguyen, T.N., Drolet-Savoie, P., Leclerc, N. & Nabi, L.R. (1998) Increased β-actin expression in an invasive Moloney sarcoma virus-transformed MDCK cell variant concentrates to the tips of multiple pseudopodia. Cancer Res. 58, 1631-1635.
8. Suzuki, H., Nagata, H., Shimada, Y. & Konno, A. (1998) Decrease in γ-actin expression, disruption of actin filaments and alterations in cell adhesion in human salivary gland adenocarcinoma cell clones. Inter. J. Oncol. 12, 1079-1084.
9. Helige, C., Zellnig, G., Hofmann-Wellenhof, R., Fink-Puches, R., Smolle, J. & Tritthart, H.A. (1997) Interrelation of motility, cytoskeletal organization and gap junctional communication with invasiveness of melanocytic cells in vitro. Invasion Metastasis 17, 26-41.
10. Verschueren, H., Van Der Taelen, I., Dewit, J., De Braekeleer, J. & De Beatselier, P. (1994) Metastatic competence of BW5147 T-lymphoma cell lines is correlated with in vitro invasiveness, motility and F-actin content. J. Leukoc. Biol. 55, 552-556.
11. Verschueren, H., Van Der Taelen, I., Dewit, J., De Braekeleer, J., De Beatselier, P., Aktories, K. & Just, I. (1995) Effects of Clostridium botulinum C2 toxin and cytochalasin D on in vitro invasiveness, motility and F-actin content of a murine T-lymphoma cell line. Eur. J. Cell Biol. 66, 335-341.
12. Iwazaki, R., Watanabe, S., Otaka, K., Ota, K., Ono, Y. & Sato, N. (1997) The role of the cytoskeleton in migration and proliferation of a cultured human gastric cancer cell line using a new metastasis model. Cancer Lett. 119, 191-196.
13. Stournaras, C., Stiakaki, E., Koukouritaki, S.B., Theodoropoulos, P.A., Kalmanti, M., Fostinis, Y. & Gravanis, A. (1996) Altered actin polymerisation dynamics in various malignant cell types: Evidence for differential sensitivity to cytochalasin B. Biochem. Pharmacol. 52, 1339-1346.
14. Malicka-Błaszkiewicz, M., Styczeń, I., Nowak, D., Hańczycowa, H., Ponikowski, P. & Sebzda, T. (1995) Actin content and polymerisation in tumour, liver and serum of the hepatoma Morris 5123 tumour bearing rats. Mat. Med. Pol. 27, 115-118.
15. Nowak, D., Majcher, I., Kochman, A. & Malicka-Błaszkiewicz, M. (1995) The changes in actin content and polymerisation during hepatoma Morris 5123 tumour development. J. Exp. Clin. Cancer Res. 14, 37-40.
16. Jordan, M.A. & Wilson, L. (1998) Microtubules and actin filaments: Dynamic targets for cancer chemotherapy. Curr. Opin. Cell Biol. 10, 123-130.
17. Bubb, M.R., Spector, I., Bershadsky, A.D. & Korn, E.D. (1995) Swinholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments. J. Biol. Chem. 270, 3463-3466.
18. Suenaga, K., Kamei, N., Okugawa, Y., Takagi, M., Akao, A., Kigoshi, H. & Yamada, K. (1997) Cytotoxicity and actin depolymerizing activity of aplyronine A, a potent antitumour macrolide of marine origin, and the natural and artificial analogs. Bioorg. Medic. Chem. Lett. 7, 269-274.
19. Jolivet, J., Cowan, K.H., Curt, G.A., Clendeninn, N.J. & Chabner, B.A. (1983) The pharmacology and clinical use of methotrexate. N. Eng. J. Med. 3, 1094-1104.
20. Takemura, Y. & Jackman, A.L. (1997) Folate-based thymidylate synthase inhibitors in cancer chemotherapy. Anti-Cancer Drug 8, 3-16.
21. Laurance, J.E. & Sandborn, W.J. (1996) Methotrexate for inflammatory bowel disease: Pharmacology and preliminary results. Mayo Clin. Proc. 71, 69-80.
22. Otrocka, M., Nowak, D., Sobczak, I. & Malicka-Błaszkiewicz, M. (1999) The effect of methotrexate (MTX) on the changes in actin content and polymerisation during hepatoma Morris 5123 growth process. Diag. Lab. 35, 399-407.
23. Morris, H.P., Sidransky, H., Wagner, B.P. & Dyer, H.M. (1960) Some characteristic of transplantable rat hepatoma 5123 induced by ingestion of N-(2-fluorenyl)phthalamic acid. Cancer Res. 20, 1252-1254.
24. Doyle, A., Griffiths, J.B. & Newell, D.G. (1993) Basic techniques for primary cell cultures and establishment of continuous cultures; in: Cell and Tissue Culture: Laboratory Procedures, chap. 3E, pp. 2.1-2.8, John Wiley & Sons, Chichester.
25. Albini, A., Iwamoto, Y., Kleinman, H.K., Martin, G.R., Aaronson, S.A., Kozlowski, J.M. & Mcewan, R.N. (1987) A rapid in vitro assay for quantitating invasive potential of tumour cells. Cancer Res. 47, 3239-3245.
26. Malicka-Błaszkiewicz, M. & Roth, J.S. (1981) Some factors affecting the interaction between actin leukemia L1210 cells and DNase I. Biochem. Biophys. Res. Commun. 102, 594-601.
27. Malicka-Błaszkiewicz, M. (1986) DNase-like activity and actin content in the liver of some vertebrates. Comp. Biochem. Physiol. 84B, 207-209.
28. Lowry, O.H., Rosebrough, N.J., Farr, A.L & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
29. Bond, M. & Somlyo, A.V. (1982) Dense bodies and actin polarity in vertebrate smooth muscle. J. Cell Biol. 95, 403-413.
30. Duncan, K., Duncan, M., Alley & M. Sausville, E. (1996) Cucurbitacin E-induced disruption of the actin and vimentin cytoskeleton in prostate carcinoma cells. Biochem. Pharmacol. 52, 1553-1560.
31. Zeng, H., Xu, Z. & Wang, K. (1997) FT-Raman studies on the transformation of G-actin to F-actin, the binding of cisplatin and transplantin to F-actin and the effect of conformation of F-actin. Int. J. Biol. Macromol. 20, 107-113.
32. Kruidering, M., van de Water, B., Zhan, Y., Baelde, J.J., de Heer, E., Mulder, G.J., Stevens, J.L. & Nagelkerke, J.F. (1998) Cisplatin effects on F-actin and matrix proteins precede renal tubular cell detachment and apoptosis in vitro. Cell Death Differ. 5, 601-614.
33. Rosenblum, M. & Shivers, R. (2000) 'Rings' of F-actin form around the nucleus in cultured human MCF7 adenocarcinoma cells upon exposure to both taxol and taxotere. Comp. Biochem. Physiol. 125, 121-131.
34. Tikoo, A., Shakri, R., Connolly, L., Hirokawa, Y., Shishido, T., Bowers, B., Ye, L., Kohama, K., Simpson, R.J. & Maruta, H. (2000) Treatment of ras-induced cancers by the F-actin-bundling drug MKT-077. Cancer J. 3, 162-168.
35. Terzis, A.J.A., Fiskerstrand, T., Refsum, H., Ueland, P.M., Arnold, H. & Bjerkvig, R. (1993) Proliferation, migration and invasion of human glioma cells exposed to antifolate drugs. Int. J. Cancer 54, 112-118.