Systematic mutational analysis of the amino-terminal domain of the Listeria monocytogenes ActA protein reveals novel functions in actin-based motility

Molecular Microbiology

Volumn 42, Issue 5, 2001, Pages 1163-1177

  Lauer, Peter ^a   Theriot, Julie A ^b   Skoble, Justin ^a   Welch, Matthew D ^a   Portnoy, Daniel A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; BACTERIAL PROTEIN; MONOMER; PROTEIN ACTA; UNCLASSIFIED DRUG;

ALLELE; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; CYTOPLASM; GENE MUTATION; LISTERIA MONOCYTOGENES; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; SEQUENCE HOMOLOGY;

ACTINS; ALANINE; AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BINDING SITES; CELL LINE; CYTOPLASM; DOGS; GREEN FLUORESCENT PROTEINS; KIDNEY; LISTERIA MONOCYTOGENES; LUMINESCENT PROTEINS; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MOVEMENT; MUTAGENESIS, SITE-DIRECTED; PHENOTYPE; RECOMBINANT FUSION PROTEINS; TRANSFECTION; VARIATION (GENETICS);

BACTERIA (MICROORGANISMS); LISTERIA; LISTERIA MONOCYTOGENES; POSIBACTERIA;

EID: 0035725729     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2001.02677.x     Document Type: Article

References (54)

1. A systematic mutational analysis of hormone-binding determinants in the human growth hormone receptor
2. Adoptive transfer of immunity to Listeria monocytogenes. The influence of in vitro stimulation on lymphocyte subset requirements
3. Expression and phosphorylation of the Listeria monocytogenes ActA protein in mammalian cells
4. Motility of ActA protein-coated micro-spheres driven by actin polymerization
5. Dual roles of plcA in Listeria monocytogenes pathogenesis
6. A focal adhesion factor directly linking intracellularly motile Listeria monocytogenes and Listeria ivanovii to the actin-based cytoskeleton of mammalian cells
7. Actin and phosphoinositide binding by the ActA protein of the bacterial pathogen Listeria monocytogenes
8. Control of actin assembly and disassembly at filament ends
9. Interactions of Listeria monocytogenes with mammalian cells during entry and actin-based movement: Bacterial factors, cellular ligands and signaling
10. Listeria monocytogenes moves rapidly through the host-cell cytoplasm by inducing directional actin assembly
11. A novel bacterial virulence gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin
12. Listeria monocytogenes, a food-borne pathogen
13. Targeting of Listeria monocytogenes ActA protein to the plasma membrane as a tool to dissect both actin-based cell morphogenesis and ActA function
14. Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions
15. Gene splicing by overlap extension: Tailor-made genes using the polymerase chain reaction
16. L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein
17. Polarized distribution of Listeria monocytogenes surface protein ActA at the site of directional actin assembly
18. The unrelated surface proteins ActA of Listeria monocytogenes and IcsA of Shigella flexneri are sufficient to confer actin-based motility on Listeria innocua and Escherichia coli respectively
19. Rapid and efficient site-specific mutagenesis without phenotypic selection
20. Efficient site-directed mutagenesis using uracil-containing DNA
21. The amino-terminal part of ActA is critical for the actin-based motility of Listeria monocytogenes; the central proline-rich region acts as a stimulator
22. Identification of two regions in the N-terminal domain of ActA involved in the actin comet tail formation by Listeria monocytogenes
23. Reconstitution of actin-based motility of Listeria and Shigella using pure proteins
24. The Arp2/3 complex: A multifunctional actin organizer
25. Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex
26. Actin-based movement of Listeria monocytogenes: Actin assembly results from the local maintenance of uncapped filament barbed ends at the bacterium surface
27. The Arp2/3 complex is essential for the actin-based motility of Listeria monocytogenes
28. 2-dependent manner downstream of tyrosine kinases
29. Stability of the Listeria monocytogenes ActA protein in mammalian cells is regulated by the N-end rule pathway
30. Expression of listeriolysin O and ActA by intracellular and extracellular Listeria monocytogenes
31. Intracellular and cell-to-cell spread of Listeria monocytogenes involves interaction with F-actin in the enterocytelike cell line Caco-2
32. ActA is a dimer
33. Localization of the ActA polypeptide of Listeria monocytogenes in infected tissue culture cell lines: ActA is not associated with actin 'comets'
34. LaXp180, a mammalian ActA binding protein, identified with the yeast two-hybrid system, co-localizes with intracellular Listeria monocytogenes
35. The ActA protein of Listeria monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton
36. Mutations of arginine residues within the 146-KKRRK-150 motif of the ActA protein of Listeria monocytogenes abolish intracellular motility by interfering with the recruitment of the Arp2/3 complex
37. Listeria monocytogenes exploits normal host cell processes to spread from cell to cell
38. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly
39. Three regions within ActA promote Arp2/3 complex-mediated actin nucleation and Listeria monocytogenes motility
40. Pivotal role of VASP in Arp2/3 complex-mediated actin nucleation, actin branch-formation and Listeria monocytogenes motility
41. Asymmetric distribution of the Listeria monocytogenes ActA protein is required and sufficient to direct actin-based motility
42. The tandem repeat domain in the Listeria monocytogenes ActA protein controls the rate of actin-based motility, the percentage of moving bacteria, and the localization of vasodilator-stimulated phosphoprotein and profilin
43. Use of a new integrational vector to investigate compartment-specific expression of the Bacillus subtilis spoIIM gene
44. Listeria monocytogenes ActA protein interacts with phosphatidylinositol 4,5-bisphosphate in vitro
45. Isolation of Listeria monocytogenes small-plaque mutants defective for intracellular growth and cell-to-cell spread
46. The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization
47. Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts
48. Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes
49. Purification and assay of the platelet Arp2/3 complex
50. Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation
51. Systematic mutational analysis of the yeast ACT1 gene
52. The Wiskott-Aldrich syndrome protein directs actin-based motility by stimulating actin nucleation with the Arp2/3 complex
53. Activation of the Arp2/3 complex by the Listeria ActA protein. ActA binds two actin monomers and three subunits of the Arp2/3 complex