Identification of two regions in the N-terminal domain of ActA involved in the actin comet tail formation by Listeria monocytogenes

EMBO Journal

Volumn 16, Issue 7, 1997, Pages 1531-1540

  Lasa, Iñigo ^b   Gouin, Edith ^b   Goethals, Mark ^a   Vancompernolle, Katia ^a   David, Violaine ^b   Vandekerckhove, Joël ^a   Cossart, Pascale ^b  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b INSTITUT PASTEUR   (France)

Author keywords

ActA; Actin polymerization; Actin tail; Intracellular movement; Listeria monocytogenes

Indexed keywords

ACTIN; ANTIBODY; BETA GALACTOSIDASE; F ACTIN;

ACTIN FILAMENT; AMINO TERMINAL SEQUENCE; ARTICLE; CELL MOTILITY; LISTERIA MONOCYTOGENES; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN VARIANT;

ACTINS; AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; BETA-GALACTOSIDASE; BINDING SITES; CELL LINE; CELL MOVEMENT; CONSERVED SEQUENCE; DNA PRIMERS; FEMALE; LISTERIA MONOCYTOGENES; MACROPHAGES; MEMBRANE PROTEINS; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OVUM; PEPTIDE FRAGMENTS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION; XENOPUS LAEVIS;

EID: 0030900504     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.7.1531     Document Type: Article

References (38)

1. Brenner,S.L. and Korn,E.D. (1983) On the mechanism of actin monomer-polymer subunit exchange at steady state. J. Biol. Chem., 258, 5013-5020.
2. Brundage,R.A., Smith,G.A., Camilli,A., Theriot,J. and Portnoy,D.A. (1993) Expression and phosphorylation of the Listeria monocytogenes ActA protein in mammalian cells. Proc. Natl Acad. Sci. USA, 90, 11890-11894.
3. Celada,F., Ullmannn,A. and Monod,J. (1974) An immunological study of complementary fragments of β-galactosidase. Biochemistry, 13, 5543-5547.
4. Chakraborty,T. et al. (1995) A focal adhesion factor directly linking intracellularly motile Listeria monocytogenes and Listeria ivanovii to the actin-based cytoskeleton of mammalian cells. EMBO J., 14, 1314-1321.
5. Cossart,P. (1995) Bacterial actin based motility. Curr. Opin. Cell Biol., 7, 94-101.
6. Cudmore,S., Cossart,P., Griffiths,G. and Way,M. (1995) Actin-based motility of vaccinia virus. Nature, 378, 636-638.
7. Domann,E., Wehland,J., Rohde,M., Pistor,S., Hartl,M., Goebel,W., Leimeister-Wächter,M., Wuenscher,M. and Chakraborty,T. (1992) A novel bacterial gene in Listeria monocytogenes required for host cell microfilament interaction with homology to the proline-rich region of vinculin. EMBO J., 11, 1981-1990.
8. Dramsi,S., Kocks,C., Forestier,C. and Cossart,P. (1993) Internalin-mediated invasion of epithelial cells by Listeria monocytogenes is regulated by the bacterial growth state, temperature and the pleiotropic activator, prfA. Mol. Microbiol., 9, 931-941.
9. Friederich,E., Gouin,E., Hellio,R., Kocks,C., Cossart,P. and Louvard,D. (1995) Targeting of Listeria monocytogenes ActA protein to the plasma membrane as a tool to dissect both actin-based cell morphogenesis and ActA function. EMBO J., 14, 2731-2744.
10. Gouin,E., Dehoux,P., Mengaud,J., Kocks,C. and Cossart,P. (1995) iactA of Listeria ivanovii although distantly related to Listeria monocytogenes actA restores actin tail formation in a L. monocytogenes actA mutant. Infect. Immun., 63, 2729-2737.
11. Hirs,C.H.W. (1967) Detection of peptides by chemical methods. Methods Enzymol., 11, 325-329.
12. Jacobson,R.H., Zhang,X.J., DuBose,R.F. and Matthews,B.W. (1994) Three-dimensional structure of β-galactosidase from E. coli. Nature, 369, 761-766.
13. Jockusch,B. and Rüdiger,M. (1996) Crosstalk between cell adhesion molecules: vinculin as a paradigm for regulation by conformation. Trends Cell Biol., 6, 311-315.
14. Kocks,C., Gouin,E., Tabouret,M., Berche,P., Ohayon,H. and Cossart,P. (1992) Listeria monocytogenes-induced actin assembly requires the actA gene product, a surface protein. Cell, 68, 521-531.
15. Kocks,C., Hellio,R., Gounon,P., Ohayon,H. and Cossart,P. (1993) Polarized distribution of Listeria monocytogenes surface protein ActA at the site of directional actin assembly. J. Cell Sci., 105, 699-710.
16. Kocks,C., Marchand,J.B., Gouin,E., d'Hauteville,H., Sansonetti,P.J., Carlier,M.F and Cossart,P. (1995) The unrelated surface proteins ActA of Listeria monocytogenes and IcsA of Shigella flexneri are sufficient to confer actin-based motility to L. innocua and E. coli respectively. Mol. Microbiol., 18, 413-423.
17. Kouyama,T. and Michashi,K. (1981) Fluorimetry study of N-(1-pyrenyl)iodoacetamide-labelled F-actin. Eur. J. Biochem., 114, 33-38.
18. Kreft,J., Dumbsky,M. and Theiss,S. (1995) The actin polymerization protein from Listeria ivanovii is a large repeat protein which shows only limited amino-acid sequence homology to ActA from Listeria monocytogenes. FEMS Microbiol. Lett., 126, 113-122.
19. Lasa,I. and Cossart,P. (1996) Actin-based motility: towards a definition of the minimal requirements. Trends Cell Biol., 6, 109-114.
20. Lasa,I., David,V., Gouin,E., Marchand,J. and Cossart,P. (1995) The amino-terminal part of ActA is critical for the actin based motility of Listeria monocytogenes; the central proline-rich region acts as a stimulator. Mol. Microbiol., 18, 425-436.
21. Lett,M.C., Sasakawa,C., Okada,N., Sakai,T., Makino,S., Yamada,M., Komatsu,K. and Yoshikawa,M. (1989) virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence. J. Bacterial., 171, 353-359.
22. Marchand,J.B., Moreau,P., Paoletti,A., Cossart,P., Carlier,M.F. and Pantaloni,D. (1995) Actin-based movement of Listeria monocytogenes in Xenopus egg extracts is due to local uncapping of the barbed ends of actin filaments at the bacterial surface and resulting shift in steady state of actin assembly. J. Cell Biol., 130, 331-343.
23. Pantaloni,D. and Carlier,M.F. (1993) How profilin promotes actin filament assembly in the presence of thymosin β4. Cell, 75, 1007-10014.
24. Pistor,S., Chakraborty,T., Niebuhr,K., Domann,E. and Wehland,J. (1994) The ActA protein of L. monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton. EMBO J., 13, 758-763.
25. Pistor,S., Chakraborty,T., Walter,U. and Wehland,J. (1995) The bacterial actin nucleator protein ActA of Listeria monocytogenes contains multiple binding sites for host microfilament proteins. Curr. Biol., 5, 517-525.
26. Pollard,T.D. and Cooper,J.A. (1986) Actin and actin-binding proteins. A critical evaluation of mechanisms and functions. Annu. Rev. Biochem., 55, 987-1035.
27. Reinhard,M., Halbrügge,M., Scheer,U., Wiegand,C., Jockusch,B.M. and Walter,U. (1992) The 45/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts. EMBO J., 11, 2063-2070.
28. Reinhard,M., Giehl,K., Abel,K., Haffner,C., Jarchau,T., Hoppe,V., Jockusch,B.M. and Walter,U. (1995) The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins. EMBO J., 14, 1583-1589.
29. Sanger,J., Mittal,B., Southwick,F.S. and Sanger,J.P. (1995) Listeria monocytogenes intracellular migration: inhibition by profilin, vitamin, D-binding protein and DNase I. Cell Motil. Cytoskeleton, 30, 38-49.
30. Smith,G.A., Portnoy,D.A. and Theriot,J.A. (1995) Asymmetric distribution of the Listeria monocytogenes ActA protein is required and sufficient to direct actin-based motility. Mol Microbiol., 17, 945-951.
31. Spudich,J.A. and Watt,S. (1971) Regulation of rabbit skeletal muscle contraction. J Biol. Chem., 246, 4866-4871.
32. Theriot,J. (1995) The cell biology of infection by intracellular bacterial pathogens. Annu. Rev. Cell Dev. Biol., 11, 213-239.
33. Theriot,J.A., Mitchison,T.J., Tilney,L.G. and Portnoy,D.A. (1992) The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization. Nature, 357, 257-260.
34. Theriot,J.A., Rosenblatt,J., Portnoy,D.A., Goldschmidt-Clermont,P.J. and Mitchison,T.J. (1994) Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts. Cell, 76, 505-517.
35. Tilney,L.G. and Tilney,M.S. (1993) The wily ways of a parasite: induction of actin assembly by Listeria. Trends Microbiol., 1, 25-31.
36. Tilney,L.G., DeRosier,D.J., Weber,A. and Tilney,M.S. (1992) How Listeria exploits host cell actin to form its own cytoskeleton. II. Nucleation, actin filament polarity, filament assembly, and evidence for a pointed end capper. J. Cell Biol., 118, 83-93.
37. Ullmannn,A., Jacob,F. and Monod,J. (1968) On the subunit structure of wild-type versus complemented β-galactosidase of Escherichia coli. J. Mol. Biol., 32, 1-32.
38. Van Troys,M., Dewitte,D., Goethals,M., Carlier,M.F., Vandekerckhove,J. and Ampe,C. (1996) The actin binding site of thymosin β-4 mapped by mutational analysis. EMBO J., 15, 201-210.