False-negative results in immunoblot assay of serum IgA antibodies reactive with the 180-kDa bullous pemphigoid antigen: The importance of primary incubation temperature

British Journal of Dermatology

Volumn 145, Issue 6, 2001, Pages 986-989

  Pas, Hendri H ^a   Kloosterhuis, G J ^a   De Jong, M C J M ^a   Jonkman, M F ^a  

^a UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

Autoimmune bullous diseases; BP180; Bullous pemphigoid; Cicatricial pemphigoid; IgA; Immunoblotting; Linear IgA dermatosis; Ocular cicatricial pemphigoid; Type XVII collagen

Indexed keywords

IMMUNOGLOBULIN A; IMMUNOGLOBULIN A ANTIBODY;

AGED; ANTIGEN ANTIBODY REACTION; ANTIGEN SPECIFICITY; ARTICLE; BASEMENT MEMBRANE; CLINICAL ARTICLE; CONTROLLED STUDY; EPIDERMIS; FEMALE; HUMAN; IMMUNOASSAY; IMMUNOBLOTTING; IMMUNOGLOBULIN BLOOD LEVEL; INCUBATION TIME; LABORATORY DIAGNOSIS; MALE; PEMPHIGOID; PRIORITY JOURNAL; RELIABILITY; ROOM TEMPERATURE; SERODIAGNOSIS; TEMPERATURE DEPENDENCE;

AGED; AGED, 80 AND OVER; ANTIBODY SPECIFICITY; ANTIGEN-ANTIBODY REACTIONS; AUTOANTIGENS; AUTOIMMUNE DISEASES; FALSE NEGATIVE REACTIONS; FEMALE; HUMANS; IMMUNOBLOTTING; IMMUNOGLOBULIN A; MALE; SKIN DISEASES, VESICULOBULLOUS; TEMPERATURE;

EID: 0035724773     PISSN: 00070963     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2133.2001.04528.x     Document Type: Article

References (13)

1. Bullous pemphigoid: Serum antibody titre and antigen specificity
2. Linear IgA bullous dermatosis with IgA antibodies exclusively directed against the 180- or 230-kDa epidermal antigens
3. Analysis of antigens targeted by circulating IgG and IgA autoantibodies in 50 patients with cicatricial pemphigoid
4. Unilateral ocular cicatricial pemphigoid with circulating IgA and IgG autoantibodies reactive with the 180 kD bullous pemphigoid antigen
5. Molecular overlap of the IgA target antigens in the subepidermal blistering diseases
6. Comparative sensitivity of indirect immunofluorescence to immunoblot assay for the detection of circulating antibodies to bullous pemphigoid antigens 1 and 2
7. Bullous pemphigoid and linear IgA dermatosis sera recognize a similar 120-kDa keratinocyte collagenous glycoprotein with antigenic cross-reactivity to BP180
8. The 97 kDa linear IgA bullous disease antigen is identical to a portion of the extracellular domain of the 180 kDa bullous pemphigoid antigen, BPAg2
9. Molecular heterogeneity of the bullous pemphigoid antigens as detected by immunoblotting
10. Patients with bullous pemphigoid and linear IgA disease show a dual IgA and IgG autoimmune response to BP180
11. Isolation of a human epidermal cDNA corresponding to the 180-kD autoantigen recognized by bullous pemphigoid and herpes gestationis sera. Immunolocalization of this protein to the hemidesmosome
12. Autoantibodies in lichen planus pemphigoides react with a novel epitope within the C-terminal NC16A domain of BP180
13. Cicatricial pemphigoid autoantibodies react with multiple sites on the BP180 extra-cellular domain