Cell-free production of biologically active polypeptides: Application to the synthesis of antibacterial peptide cecropin

Protein Expression and Purification

Volumn 21, Issue 3, 2001, Pages 456-461

  Martemyanov, Kirill A ^a   Shirokov, Vladimir A ^a   Kurnasov, Oleg V ^a   Gudkov, Anatoly T ^a   Spirin, Alexander S ^a  

^a INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0035717170     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1400     Document Type: Article

References (26)

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7. High-level expression of cecropin CMIV in E. coli from a fusion construct containing the human tumour necrosis factor
8. Expression of cecropin CMIV fusion protein in E. coli under T7 promoter
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12. A semicontinuous prokaryotic coupled transcription/translation system using a dialysis membrane
13. A continuous cell-free translation system capable of producing polypeptides in high yield
14. Cell-free protein synthesis bioreactor
15. FACS-optimized mutants of the green fluorescent protein (GFP)
16. Improved green fluorescent protein by molecular evolution using DNA shuffling
17. Green fluorescent protein as a marker for gene expression
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22. Ultrasensitive assay for endogenous antimicrobial polypeptides
23. Renaturation of a single-chain immunotoxin facilitated by chaperones and protein disulfide isomerase
24. Recent advances in producing and selecting functional proteins by using cell-free translation