Validation of confocal laser scanning microscopy for detecting intracellular calcium heterogeneity in liver slices

Journal of Pharmacological and Toxicological Methods

Volumn 45, Issue 3, 2001, Pages 187-193

  Yoneyama, Keiichiro ^a  

^a SHOWA UNIVERSITY   (Japan)

Author keywords

Confocal laser scanning microscope; Hypoxia; Intracellular calcium; Lobular heterogeneity; Rat liver slices

Indexed keywords

CALCIUM ION;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; AUTOFLUORESCENCE; CALCIUM CELL LEVEL; CELL HETEROGENEITY; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; HYPOXIA; LIVER SLICE; MALE; METABOLIC INHIBITION; NONHUMAN; RAT; TISSUE CULTURE; VALIDATION PROCESS;

ANIMALS; CALCIUM; CELL COUNT; CELL HYPOXIA; FLUORESCENT DYES; HEPATOCYTES; HETEROCYCLIC COMPOUNDS, 3-RING; LIVER; MALE; MICROSCOPY, CONFOCAL; RATS; RATS, WISTAR; SODIUM;

EID: 0035678526     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1056-8719(01)00147-2     Document Type: Article

References (39)

1. O-Phthalaldehyde: Fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin
2. Liver cell heterogeneity: Functions of non-parenchymal cells
3. 2+ exchange in hypoxic, ischemic, and reperfused rat hearts
4. Effects of exogenous superoxide anion and nitric oxide on the scavenging function and electron microscopic appearance of the sinusoidal endothelium in the isolated, perfused rat liver
5. The general & cellular basis of medical physiology
6. Effects of osmotic stresses on isolated rat hepatocytes II. Modulation of intracellular pH
7. Biocarbonate-dependent and -independent intracellular pH regulatory mechanisms in rat hepatocytes
8. On the uptake of materials by the intact liver. The transport and net removal of galactose
9. Peripotal and pericentral pyridine nucleotide fluorescence from the surface of the perfused liver: Evaluation of the hypothesis that chronic treatment with ethanol produces pericentral hypoxia
10. Metabolic heterogeneity of the liver
11. Functional and structural zonal hepatocyte heterogeneity - Dynamics and ontogenic development
12. Different changes in expression and function of connexin26 and connexin32 during DNA synthesis and redifferentiation in primary rat hepatocytes using a DMSO culture system
13. 2+ measured by confocal laser-scanning microscopy in bullfrog sympathetic ganglion cells
14. Topographical heterogeneity of glutamate agonist-induced calcium increase in hippocampus
15. Hepatic enzyme zonation: A reevaluation of the concept of the liver acinus
16. Spiral calcium wave propagation and annihilation in Xenopus leavis oocytes
17. Ultrastructure of the intial stages of hepatic perivenular fibrosis after alcohol
18. 2+ signals in axons of basket celles from rat cerebellar slices
19. Effects of hypoxia and metabolic inhibition on the intracellular sodium activity of mammalian ventricular muscle
20. ++ exchanger and myocardial ischemia/reperfusion
21. 2+ exchange in isolated guinea-pig cardiac myocytes
22. Perivenular fibrosis in alcoholic liver injury: Ultrastructure and histologic progression
23. Some dynamic aspects of the hepatic microcircuration - Demonstration of sinusoidal endothelial fenestrae as a possible regulatory factor
24. Store depletion and calcium influx
25. The identification of phenylalamine on paper chromatograms
26. Subdivision of hexagonal liver lobules into a structural and functional unit: Role in hepatic physiology and pathology
27. Physioanatomic considerations
28. Kupffer cell inactivation prevents lipopolysaccharide-induced structural changes in the rat liver sinusoid: An electron-microscopic study
29. Differential lobular induction in rat liver of glutathione S-transferase A1/A2 by phenobarbital
30. Dynamic organ culture of precision liver slices for in vitro toxicology
31. The hepatocytes-specific phenotype of murine liver cells correlates with high expression of connxin32 and connexin26 but very low expression of connexin43
32. Regionality of glucose-6-phosphate hydrolysis in the liver lobule of the rat: Metabolic heterogeneity of "portal" and "septal" sinusoids
33. Distribution of 3-hydroxybutyrate dehydrogenase in primary lobules of rat liver
34. Three-dimensional reconstruction of parenchymal units in the liver of the rat
35. 2+ using rat liver tissue
36. Isolation and enrichment of two sublobular compartment-specific endothelial cell subpopulations from liver sinusoids
37. The liver sieve: Considerations concerning the structure and function of endothelial fenestrae, the sinusoidal wall and the space of Disse
38. Demonstration by in situ hybridization of the zonal modulation of rat liver cytochrome P-450b and P-450e gene expression after phenobarbital
39. Zonal and regional differences identified from precision mapping of vitamin A-storing lipid droplets of the hepatic stellate cells in pig liver: A novel concept of addressing the intralobular area of heterogeneity