Adrenodoxin-cytochrome P450scc interaction as revealed by EPR spectroscopy: Comparison with the putidaredoxin-cytochrome P450cam system

Journal of Biochemistry

Volumn 130, Issue 6, 2001, Pages 789-797

  Takeuchi, Kohji ^a   Tsubaki, Motonari ^a,b   Futagawa, Junya ^b   Masuya, Futoshi ^b   Hori, Hiroshi ^b  

^a UNIVERSITY OF HYOGO   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Adrenodoxin; Cytochrome P450cam; Cytochrome P450scc; EPR; Putidaredoxin

Indexed keywords

ADRENODOXIN; CAMPHOR 5 MONOOXYGENASE; CARBON MONOXIDE; CHOLESTEROL; CHOLESTEROL MONOOXYGENASE (SIDE CHAIN CLEAVING); FERREDOXIN; HEME; IRON; NITRIC OXIDE; PREGNENOLONE; PUTIDAREDOXIN; STEROID; SULFUR; UNSPECIFIC MONOOXYGENASE;

ARTICLE; CATALYST; CHOLESTEROL SIDE CHAIN CLEAVAGE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ELECTRON SPIN RESONANCE; HYDROXYLATION; MOLECULAR INTERACTION; NONHUMAN; OXIDATION; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PSEUDOMONAS; REDUCTION; STOICHIOMETRY;

DRYOBALANOPS; PSEUDOMONAS;

EID: 0035660286     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003050     Document Type: Article

References (44)

1. Cytochrome P-450 enzymes in sterol biosynthesis and metabolism
2. Ionic effects on adrenal steroidogenic electron transport. The role of adrenodoxin as an electron shuttle
3. scc
4. scc
5. scc and inhibits adrenodoxin binding
6. Molecular cloning and nucleotide sequence of cDNA for mRNA of mitochondrial cytochrome P-450(SCC) of bovine adrenal cortex
7. Molecular cloning and nucleotide sequence of DNA of mitochondrial cytochrome P-450(11Β) of bovine adrenal cortex
8. 3 25-hydroxylase from rat liver mitochondria
9. 3 1α-hydroxylase and vitamin D synthesis
10. 3 24-hydroxylase cDNA
11. Identification by site-directed mutagenesis of two lysine residues in cholesterol side chain cleavage cytochrome P450 that are essential for adrenodoxin binding
12. An additional electrostatic interaction between adrenodoxin and P450c27 (CYP27A1) results in tighter binding than between adrenodoxin and P450scc (CYP11A1)
13. scc-phenyl isocyanide complex upon binding of reduced adrenodoxin
14. scc-nitric oxide complexes: Effects of 20(R),22(R)-dihydroxycholesterol and reduced adrenodoxin
15. scc: Implications of the productive and nonproductive substrate bindings
16. Putidaredoxin-cytochrome P450cam interaction. Spin state of the heme iron modulates putidaredoxin structure
17. scc: Chemical identification of the heme-binding cysteine residue
18. Heterogeneity of adrenocortical ferredoxin
19. scc-nitric oxide complexes: Effects of cholesterol and its analogues
20. Uncoupling of the cytochrome P-450cam monooxygenase reaction by a single mutation, threonine-252 to alanine or valine: A possible role of the hydroxy amino acid in oxygen activation
21. 561 from ascorbate but does not influence on electron donation to monodehydroascorbate radical: Distinct roles of two heme centers for electron transfer across the chromaffin vesicle membranes
22. Steroid binding properties of beef adrenal cortical cytochrome P-450 which catalyzes the conversion of cholesterol into pregnenolone
23. cam
24. Adrenodoxin with a COOH-terminal deletion (des 116-128) exhibits enhanced activity
25. Molecular cloning and amino acid sequence of the precursor form of bovine adrenodoxin: Evidence for a previously unidentified COOH-terminal peptide
26. scc: A resonance Raman study
27. cam and putidaredoxin
28. 112 residue of cytochrome P450cam for electron transfer from reduced putidaredoxin
29. cam. Effects of d-camphor and putidaredoxin on the iron-ligand structure
30. An anomalous Fe-CO stretching vibration for the CO complex of cytochrome P450cam
31. An NMR-derived model for the solution structure of oxidized putidaredoxin, a 2-Fe, 2-S ferredoxin from Pseudomonas
32. 1H NMR spectral features and tertiary structural constraints on the C-terminal region of putidaredoxin
33. New aspects of electron transfer revealed by the crystal structure of a truncated bovine adrenodoxin, Adx(4-108)
34. The tertiary structure of full-length bovine adrenodoxin suggests functional dimers
35. C-terminal region of aderenodoxin affects its structural integrity and determines differences in its electron transfer function to cytochrome P-450
36. A structure-based model for cytochrome P450cam-putidaredoxin interactions
37. scc from bovine adrenocortical mitochondria
38. cam on binding of specific substrates
39. cam complexed with putidaredoxin
40. Steroidogenic electron transport in adrenal cortex mitochondria
41. scc with adrenodoxin and adrenodoxin reductase-adrenodoxin complex. The implication in ACTH function
42. scc in Cytochrome P450
43. Adrenodoxin reductase-adrenodoxin complex structure suggests electron transfer path in steroid biosynthesis
44. Covalently crosslinked complexes of bovine adrenodoxin with adrenodoxin reductase and cytochrome P450scc. Mass spectrometry and Edman degradation of complexes of the steroidogenic hydroxylase system